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DEVELOPMENT OF PARACEST MRI TO DETECT CANCER BIOMARKERS

Liu, Guanshu
http://rave.ohiolink.edu/etdc/view?acc_num=case1199932704

Abstract Details

2008, Doctor of Philosophy, Case Western Reserve University, Biomedical Engineering.
Molecular imaging has become one of the most significant developments in MRI in the last decade with dramatic impacts for disease diagnosis and therapy. A novel MRI contrast strategy named PARAmagnetic Chemical Exchange Saturation Transfer(PARACEST) can exploit the NMR chemical shift to selectively detect molecular biomarkers, while retaining the sensitivity and spatial resolution of MRI. The development of this novel approach for cancer biomarker detection is described in the following chapters. Chapter 1: Background information is presented about the relevance of biomarker detection by molecular imaging and MRI molecular imaging. The mechanisms of MRI contrast agent including T 1contrast agents, T 2/T 2* contrast agents and CEST/PARACEST contrast agents are introduced. The PARACEST contrast mechanism is then emphasized for the potential biomedical applications. Chapter 2: A new irreversible nitric oxide (NO) responsive PARACEST MRI contrast agent, Yb-DO3oAA, has been designed, synthesized and characterized. The PARACEST effects have been investigated with respect to pH, temperature, and concentration for the in vivoapplicability. The ability to detect NO has been demonstrated in vitro. Chapter 3: A new MRI method has been developed for assessing in vivo pH by using a PARACEST MRI contrast agent Yb-DO3AoAA. A ratiometric approach has been employed based on the two intra-molecular PARACEST signals. Our study has demonstrated that this method can be used for measuring extracellular pH within in vivo animal models without the need for a second “control” agent. Chapter 4: New MRI pulse sequences have been developed to address the poor temporal resolution challenges for the in vivoapplications of PARACEST agents. Different strategies have been developed for the applications in different in vivo environments. These new MRI methods have been developed for high field small animal studies and tested both within in vitroand in vivomodels. Chapter 5: The major drawbacks and technical hurdles in PARACEST studies are presented and followed by the discussion of future developments. A number of potential projects thereby are proposed as future studies.
Mark Pagel (Advisor)
192 p.
Engineering, Biomedical
MRI; molecular imaging; contrast agent; PARACEST; cancer biomarker

Recommended Citations

Citations

  • Liu, G. (2008). DEVELOPMENT OF PARACEST MRI TO DETECT CANCER BIOMARKERS [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1199932704

    APA Style (7th edition)

  • Liu, Guanshu. DEVELOPMENT OF PARACEST MRI TO DETECT CANCER BIOMARKERS. 2008. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1199932704.

    MLA Style (8th edition)

  • Liu, Guanshu. "DEVELOPMENT OF PARACEST MRI TO DETECT CANCER BIOMARKERS." Doctoral dissertation, Case Western Reserve University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1199932704

    Chicago Manual of Style (17th edition)

case1199932704
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© 2008, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.