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A Nonlinear Response Model for Single Nucleotide Polymorphism Detection Assays

Kouri, Drew P.
http://rave.ohiolink.edu/etdc/view?acc_num=case1212598582

Abstract Details

2008, Master of Sciences, Case Western Reserve University, Mathematics.
Malaria is a significant cause of mortality in the tropical regions of the world, such as Papua New Guinea (PNG). Efforts to combat malaria are impeded by the development of drug resistant mutants. We generated a model describing the chemical and molecular properties of the ligase detection reaction (LDR) fluorescent microsphere assay (FMA) for single nucleotide polymorphism (SNP) detection and employed numerical optimization techniques to determine the parameters of this model. First, we implemented the Levenberg-Marquardt Nonlinear Least Squares (NLLS) algorithm and estimated the model parameters from simulated data representing a control dilution/mixing experiment. Second, we used these parameters as well as parameters estimated from experimental data to generate possible distributions of parasite concentration. These distributions can, in principle, inform us of how drug resistance is distributed in a given sample and throughout PNG communities in general. Furthermore, they allow us to evaluate a drug's effectiveness in that community.
Peter Thomas, PhD (Committee Chair)
Daniela Calvetti, PhD (Committee Member)
Peter Zimmerman, PhD (Committee Member)
James Alexander, PhD (Committee Member)
111 p.
Mathematics
xc; Trust Region; del; ¿¿¿¿; LDR

Recommended Citations

Citations

  • Kouri, D. P. (2008). A Nonlinear Response Model for Single Nucleotide Polymorphism Detection Assays [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1212598582

    APA Style (7th edition)

  • Kouri, Drew. A Nonlinear Response Model for Single Nucleotide Polymorphism Detection Assays. 2008. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1212598582.

    MLA Style (8th edition)

  • Kouri, Drew. "A Nonlinear Response Model for Single Nucleotide Polymorphism Detection Assays." Master's thesis, Case Western Reserve University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1212598582

    Chicago Manual of Style (17th edition)

case1212598582
582
© 2008, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.