Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


case1216141039.pdf (430.59 KB)

ETD Abstract Container

Abstract Header

Assessing the Role of Glyceroneogenesis in Triglyceride Metabolism

Nye, Colleen Klocek
http://rave.ohiolink.edu/etdc/view?acc_num=case1216141039

Abstract Details

2008, Doctor of Philosophy, Case Western Reserve University, Biochemistry.
The formation of triglyceride in mammalian tissues requires the provision of glycerol-3-phosphate as the source of triglyceride glycerol. We have quantified the relative contribution of glyceroneogenesis and glycolysis to triglyceride glycerol synthesis in vivo in adipose tissue, skeletal muscle, and liver of the rat in response to a chow diet (controls), 48 h of fasting, and lipogenic (high sucrose) diet. The rate of glyceroneogenesis was quantified using the tritium ([3H2]O) labeling of body water method, and the contribution of glucose, via glycolysis, was determined using [U-14C]glucose tracer. In epididymal and mesenteric fat of control rats, glyceroneogenesis accounted for ~90% of triglyceride glycerol synthesis. Fasting for 48 h did not alter glyceroneogenesis in adipose tissue, whereas the contribution of glucose was negligible. In response to sucrose feeding, the synthesis of triglyceride glycerol via both glyceroneogenesis and glycolysis nearly doubled (vs. controls); however, glyceroneogenesis remained quantitatively higher as compared to the contribution of glucose. Enhancement of triglyceride-fatty acid cycling by epinephrine infusion resulted in a higher rate of glyceroneogenesis in adipose tissue as compared with controls, while the contribution of glucose, via glycolysis, was not measurable. Glyceroneogenesis provided the majority of triglyceride glycerol in the gastrocnemius and soleus muscle. In the liver, the fractional contribution of glyceroneogenesis remained constant (~60%) under all conditions and was higher than that of glucose. Thus, glyceroneogenesis, in contrast to glucose, via glycolysis, is quantitatively the predominant source of triglyceride glycerol in adipose tissue, skeletal muscle, and liver of the rat under conditions ranging from extended fasting to high sucrose feeding.
Richard Hanson, PhD (Advisor)
Satish Kalhan, MD (Advisor)
William Merrick, PhD (Committee Chair)
Martin Snider, PhD (Committee Member)
Arthur McCullough, MD (Committee Member)
116 p.
Biochemistry
glyceroneogenesis; glycerol-3-phosphate; triglyceride glycerol; glycolysis; in vivo; isotope tracer; quantify

Recommended Citations

Citations

  • Nye, C. K. (2008). Assessing the Role of Glyceroneogenesis in Triglyceride Metabolism [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1216141039

    APA Style (7th edition)

  • Nye, Colleen. Assessing the Role of Glyceroneogenesis in Triglyceride Metabolism. 2008. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1216141039.

    MLA Style (8th edition)

  • Nye, Colleen. "Assessing the Role of Glyceroneogenesis in Triglyceride Metabolism." Doctoral dissertation, Case Western Reserve University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1216141039

    Chicago Manual of Style (17th edition)

case1216141039
2,254
© 2008, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.