Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


case1238452739.pdf (6.73 MB)

ETD Abstract Container

Abstract Header

Role of Circulating Peripheral Blood-Derived Endothelial Colony-Forming Cells in Patients with Proliferative Diabetic Retinopathy

Tan, Kevin S.
http://rave.ohiolink.edu/etdc/view?acc_num=case1238452739

Abstract Details

2009, Master of Sciences, Case Western Reserve University, Clinical Research.
Proliferative diabetic retinopathy (PDR) is one of the leading causes of visual loss in the developing world. Retinal ischemia resulting from capillary occlusion is the initiating step for pre-retinal neovascularization. There is emerging evidence suggesting that circulating endothelial progenitor cells participate in post-natal neoangiogenesis and specifically that CD34+ CD45- endothelial colony-forming cells (ECFCs) may have unique characteristics to form functional vessels de novo. The purpose of this study was to determine whether PDR ECFCs are impaired in their ability to repair damaged retinal vasculature. Mononuclear cells isolated from PDR patients demonstrated a two-fold increase in circulating CD34+ CD45- ECFCs compared to controls. However, PDR ECFCs were impaired in migrating towards SDF-1 and human sera and were unable to incorporate with human retinal endothelial cells into vascular tubes. PDR ECFCs expressed higher levels of thrombospondin-1 and TIMP-3. Our results suggest that ECFCs in PDR patients are mobilized into the circulation but are defective in their ability to migrate and repair damaged capillary endothelium. This may result from derangements in the expression of angiogenesis inhibitors such as thrombospondin-1 and TIMP-3.
Bela Anand-Apte, MD, PhD (Advisor)
Jonathan Sears, MD (Committee Chair)
Serpil Erzurum, MD (Committee Member)
Barbara Cromer, MD (Committee Member)
54 p.
Biomedical Research
Diabetic retinopathy; Proliferative diabetic retinopathy; Diabetes; Endothelial progenitor cells; stem cells; Endothelial colony forming cells

Recommended Citations

Citations

  • Tan, K. S. (2009). Role of Circulating Peripheral Blood-Derived Endothelial Colony-Forming Cells in Patients with Proliferative Diabetic Retinopathy [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1238452739

    APA Style (7th edition)

  • Tan, Kevin. Role of Circulating Peripheral Blood-Derived Endothelial Colony-Forming Cells in Patients with Proliferative Diabetic Retinopathy. 2009. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1238452739.

    MLA Style (8th edition)

  • Tan, Kevin. "Role of Circulating Peripheral Blood-Derived Endothelial Colony-Forming Cells in Patients with Proliferative Diabetic Retinopathy." Master's thesis, Case Western Reserve University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=case1238452739

    Chicago Manual of Style (17th edition)

case1238452739
634
© 2009, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.