Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

Impaired Hepatic Fatty Acid Synthesis: A Potential Mechanism of the Reduced Growth Phenotype of Cystic Fibrosis Knockout Mice

Bragg, Sarah A.

Abstract Details

2010, Master of Sciences, Case Western Reserve University, Genetic Counseling.
Cystic Fibrosis is a genetic disease that results in poor growth manifesting as a reduced fat mass. This study characterized the de novo fatty acid synthesis of both Cftr-null mice and hepatocyte specific Cftr knockouts to determine if this is a potential mechanism resulting in this reduced fat mass. De novo synthesis rates of palmitate and stearate were measured in the liver via isotope labeling of water and mass spectrometry. In addition, the expression of ACC-1 and AMPk were determined via western blotting. Results indicated decreased hepatic fat synthesis in CF males compared to CF females and controls. Enzyme expression results revealed differences in hepatic ACC-1 and AMPk activity levels in both CF genders, which were overall inconclusive. Hepatocyte knockouts showed no difference in palmitate or stearate synthesis and a significant increase in male AMPk expression. Overall, this study supports the notion of an impaired lipid metabolism in CF mice.
Mitchell Drumm, PhD (Advisor)
Anne Matthews, RN, PhD (Committee Member)
Shawn McCandless, MD, PhD (Committee Member)
Craig Hodges, PhD (Committee Member)
69 p.

Recommended Citations

Citations

  • Bragg, S. A. (2010). Impaired Hepatic Fatty Acid Synthesis: A Potential Mechanism of the Reduced Growth Phenotype of Cystic Fibrosis Knockout Mice [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1272503069

    APA Style (7th edition)

  • Bragg, Sarah. Impaired Hepatic Fatty Acid Synthesis: A Potential Mechanism of the Reduced Growth Phenotype of Cystic Fibrosis Knockout Mice. 2010. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1272503069.

    MLA Style (8th edition)

  • Bragg, Sarah. "Impaired Hepatic Fatty Acid Synthesis: A Potential Mechanism of the Reduced Growth Phenotype of Cystic Fibrosis Knockout Mice." Master's thesis, Case Western Reserve University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1272503069

    Chicago Manual of Style (17th edition)