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A CEST MRI METHOD TO MEASURE pH

Sheth, Vipul Ravindra
http://rave.ohiolink.edu/etdc/view?acc_num=case1290287480

Abstract Details

2011, Doctor of Philosophy, Case Western Reserve University, Biomedical Engineering.
Tumor pH is an important biomarker in cancer. In the following chapters an approach to measure pH by Chemical Exchange Saturation Transfer (CEST) MRI is described. Chapter 1 presents and introduction to molecular imaging, the importance of tumor pH and existing methods of pH measurement by various imaging modalities. Chapter 2 describes the characterization of a PARACEST MRI contrast agent, Yb-DO3A-oAA, for pH measurement. The pH measurement is independent of concentration and T1sat relaxation times, and covers a wider pH measurement range than alternative methods. A new method for fitting PARACEST spectra is shown. Chapter 3 demonstrates the self calibrating nature of CEST MRI with the contrast agent, Yb-DO3A-oAA-TML-ester, to detect esterase enzyme activity in solution and in cell culture media by generating an enzyme-responsive CEST effect that was compared with a control CEST effect from the same agent. In Chapter 4, a new MRI method was developed to simplify MRI acquisition of CEST spectra. Arrayed CEST-FISP and the parameters of this method were optimized for use with PARACEST agents. The arrayed CEST-FISP pulse program simplifies acquisition setup and subsequent analysis, and has strong potential to increase CEST experimental throughput that will facilitate future discoveries. Chapter 5 translates Yb-DO3A-oAA from in vitro to in vivo use to measure pH in a MDA-MB-231 tumor model and mouse muscle. The pharmacodynamics of Yb-DO3A-oAA are investigated and the fitting algorithm described in Chapter 2 is extended to in vivo tissues.
Zheng-Rong Lu, PhD (Committee Chair)
Christopher Flask, PhD (Committee Member)
Vikas Gulani, MD. PhD (Committee Member)
Roger Marchant, PhD (Committee Member)
Mark Pagel, PhD (Advisor)
169 p.
Biomedical Engineering
MRI; CEST; PARACEST; Paramagnetic; Saturation; pH; Magnetic Resonance; Chemical Exchange Saturation Transfer; Magnetic Resonance Imaging; Lanthanide

Recommended Citations

Citations

  • Sheth, V. R. (2011). A CEST MRI METHOD TO MEASURE pH [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1290287480

    APA Style (7th edition)

  • Sheth, Vipul. A CEST MRI METHOD TO MEASURE pH. 2011. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1290287480.

    MLA Style (8th edition)

  • Sheth, Vipul. "A CEST MRI METHOD TO MEASURE pH." Doctoral dissertation, Case Western Reserve University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1290287480

    Chicago Manual of Style (17th edition)

case1290287480
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© 2010, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.