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Optode-bead-based Functional Chemical Imaging of 2D Substrates

Ahuja, Punkaj N.
http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754

Abstract Details

2011, Master of Engineering, Case Western Reserve University, Biomedical Engineering.
We introduce a new technology for functional imaging of surfaces using microscopic optode beads. The imaging technology produces a map of 2D chemical distribution, obtained continuously and simultaneously at each pixel using a charged coupled device (CCD) camera. Ionic distributions can be imaged with this technique using ionophore-chromoionophores interactions in microscopic optode beads or membranes. Response to pH diffusion from a 140 um channel were measured. Lateral and spherical diffusion processes are compared. Data was resolved at each pixel using linear interpolation of three closest beads with Delaunay triangulation and color- concentration maps were created assuming circular symmetry. The approach is similar to Scanning Electrochemical Microscopy, an imaging modality that relies on the scanning of an electrochemical microsensor along a surface of interest. Potential or current measures local concentrations of an analyte at each scanned position. Scanning of the microelectrode tip is, however, time consuming and the tip itself may run into physical obstruction at uneven surfaces. Functional optode-bead imaging provides an imaging modality that can produce similar functional 2D maps as scanning electrochemical microscopy does, but without the need for physical scanning of an electrode. The functional optode bead technology has a variety of potential including measurements in systems scanning electrochemical microscopy could not be applied. Some biomedical applications include imaging of pH in epithelial cell monolayers and multicellular tumor spheroids (MCTS), which are meant to represent tumor microenvironments.
Miklos Gratzl, PhD (Committee Chair)
Andrew Rollins, PhD (Committee Member)
Shawn McCandless, MD (Committee Member)
Biomedical Engineering
Imaging; electrochemical; electrochemistry; scanning electrochemical microscopy; secm; ion; pH; proton; multicellular tumor spheroids; MCTS; bulk optode

Recommended Citations

Citations

  • Ahuja, P. N. (2011). Optode-bead-based Functional Chemical Imaging of 2D Substrates [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754

    APA Style (7th edition)

  • Ahuja, Punkaj. Optode-bead-based Functional Chemical Imaging of 2D Substrates. 2011. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754.

    MLA Style (8th edition)

  • Ahuja, Punkaj. "Optode-bead-based Functional Chemical Imaging of 2D Substrates." Master's thesis, Case Western Reserve University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1307727754

    Chicago Manual of Style (17th edition)

case1307727754
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© 2011, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.