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Visualization of the ribbon synapse using Ribeye a-mCherry fusion protein

West, Megan C.
http://rave.ohiolink.edu/etdc/view?acc_num=case1311798467

Abstract Details

2011, Master of Sciences, Case Western Reserve University, Biology.
Specialized presynaptic matrices known as ribbon synapses are found in sensory cells of the visual, auditory, and vestibular systems of vertebrates. Hair cells of the auditory system and the vestibular system rely on ribbon synapses to transmit signals to the brain. Due to the lack of tools to visualize the ribbon synapse, studying the ribbon has been challenging. We designed the construct, Tg(pvalb3b:ribeye a-mCherry), which allows for visualization of ribbon synapses in the hair cells of live or fixed zebrafish. We show that the Ribeye a-mCherry fusion protein expressed from this construct localizes to appropriate areas using immunolabeling and live animal imaging. These results demonstrate that Tg(pvalb3b:ribeye a-mCherry) is an effective tool to label ribbons. We utilize this tool to characterize mutant zebrafish and to investigate ribbon dynamics. Our results show that Ribeye a-mCherry has the ability to be used in mutant zebrafish and in fluorescent recovery after photobleaching experiments.
Brian McDermott (Advisor)
Heather Broihier (Committee Member)
Roy Ritzmann (Committee Member)
Biology; Neurosciences
hair cell; ribbon synapse; zebrafish; hearing; Ribeye

Recommended Citations

Citations

  • West, M. C. (2011). Visualization of the ribbon synapse using Ribeye a-mCherry fusion protein [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1311798467

    APA Style (7th edition)

  • West, Megan. Visualization of the ribbon synapse using Ribeye a-mCherry fusion protein. 2011. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1311798467.

    MLA Style (8th edition)

  • West, Megan. "Visualization of the ribbon synapse using Ribeye a-mCherry fusion protein." Master's thesis, Case Western Reserve University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1311798467

    Chicago Manual of Style (17th edition)

case1311798467
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© 2011, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.