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Targeted mutagenesis of zebrafish hearing-related genes using ZFN and TALEN

Liu, Li
http://rave.ohiolink.edu/etdc/view?acc_num=case1385420140

Abstract Details

2014, Master of Sciences, Case Western Reserve University, Biology.
Hair cells are specialized mechanosensory cells with stereocilia protruding from their apical surfaces. Deflection of stereocilia allows the opening of mechanotransduction channels on the tips of the stereocilia. However, regulation of stereocilia development at the molecular level and the identity of the mechanotransduction channel remain largely unknown. Engineered ZFN and TALEN have shown to be efficient genome editing tools among many different organisms. We designed ZFN or TALEN targeting two of our genes of interest: fascin 2a and piezo 2-4 in zebrafish. The ZFNs and TALENs were constructed and successfully induced somatic mutations at the targeted loci for both genes. Founder fish that carry and pass mutation to their offspring were also found for the piezo 2-4. With the success in introducing insertion/deletion mutations within the desired target loci, we are now able to investigate the function of a gene in hair cell genesis and mechanotransduction in zebrafish.
Brian McDermott (Advisor)
Claudia Mizutani (Committee Member)
Emmitt Jolly (Committee Member)
Jean Burns (Committee Chair)
Biology
zebrafish; hair cell; ZFN; TALEN

Recommended Citations

Citations

  • Liu, L. (2014). Targeted mutagenesis of zebrafish hearing-related genes using ZFN and TALEN [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1385420140

    APA Style (7th edition)

  • Liu, Li. Targeted mutagenesis of zebrafish hearing-related genes using ZFN and TALEN. 2014. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1385420140.

    MLA Style (8th edition)

  • Liu, Li. "Targeted mutagenesis of zebrafish hearing-related genes using ZFN and TALEN." Master's thesis, Case Western Reserve University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1385420140

    Chicago Manual of Style (17th edition)

case1385420140
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© , some rights reserved.Creative Commons License Targeted mutagenesis of zebrafish hearing-related genes using ZFN and TALEN by Li Liu is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.