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ETD Abstract Container

Abstract Header

Dynamic Intravital Imaging of Immune Cells During the Initiating Events of Experimental Autoimmune Encephalomyelitis

Sim, Deborah
http://rave.ohiolink.edu/etdc/view?acc_num=case1396560202

Abstract Details

2014, Doctor of Philosophy, Case Western Reserve University, Biomedical Engineering.
Experimental autoimmune encephalomyelitis (EAE) is a murine model of central nervous system (CNS) inflammatory disease resulting in immune cell accumulation, neuronal tissue destruction and paralysis. With this work, we endeavor to reconcile the literature on EAE, from the action of pertussis toxin (PTx) in the initiation of inflammation through to infiltration of CD4+ T cells. We show for the first time that localized, transient vessel leak initiates an inflammatory state within the CNS that results in microglia activation, and recruitment of dendritic cells (DCs). We also show for the first time that microglia are able to project extensions into the blood vessel with an intact blood brain barrier under non-inflammatory conditions and that the number of projections increase during the first 12 days of EAE induction. These resident microglia and peripheral DCs accumulate near vessel leaks and recruit myelin-specific T cells to cause subsequent tissue damage and plaque formation. This inflammatory process, serial immune cell infiltration and tissue destruction can be dampened by the use of anti-histamines to inhibit the activation of microglia at the onset of disease initiation.
Alex Huang (Advisor)
180 p.
Immunology; Neurosciences

Recommended Citations

Citations

  • Sim, D. (2014). Dynamic Intravital Imaging of Immune Cells During the Initiating Events of Experimental Autoimmune Encephalomyelitis [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1396560202

    APA Style (7th edition)

  • Sim, Deborah. Dynamic Intravital Imaging of Immune Cells During the Initiating Events of Experimental Autoimmune Encephalomyelitis. 2014. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1396560202.

    MLA Style (8th edition)

  • Sim, Deborah. "Dynamic Intravital Imaging of Immune Cells During the Initiating Events of Experimental Autoimmune Encephalomyelitis." Doctoral dissertation, Case Western Reserve University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1396560202

    Chicago Manual of Style (17th edition)

case1396560202
440
© 2014, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.