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Anderson 2014.pdf (3.69 MB)

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Subthreshold Oscillations and Persistent Activity Modulate Spike Output in the Rodent Dentate Gyrus

Anderson, Ross William
http://rave.ohiolink.edu/etdc/view?acc_num=case1415229722

Abstract Details

2015, Doctor of Philosophy, Case Western Reserve University, Physiology and Biophysics.
It is known that the hippocampus is important in learning, memory and spatial navigation. How it performs these tasks at the cellular level in response to neurotransmitters and oscillations in neural circuitry is poorly understood. To determine the role of subthreshold oscillations and persistent activity in information processing in cellular circuits within the hippocampus, I chose two different methods. First, I used whole cell recordings in rat hippocampal slices in vitro to elucidate the role of cholinergic modulation on hippocampal cells. Cholinergic modulation causes dynamic changes in neuronal circuits through the release of the neurotransmitter acetylcholine. I found that cholinergic modulation within the dentate gyrus increased the excitability of dentate mossy cells but not local interneurons through activation of an M1 receptor. During cholinergic modulation, plateau potentials and persistent activity could be stabilized or completely turned off by inhibitory potentials generated by activation of local interneurons. Second, I used in vivo whole cell recordings in awake head-fixed mice to investigate subthreshold oscillations in dentate gyrus cells to determine their response during animal movement. I found that dentate gyrus neurons demonstrated subthreshold oscillations in the 8-15 Hz frequency band that were initiated near the onset of spontaneous bouts of motion. These oscillations often preceded the movement onset and their strength in the first 2-seconds was largest in movements along the animals natural forward/backward direction. Lastly, the power of this oscillation would predict the duration of time the animal would run. This data suggest that dynamic changes in network excitability as the result of cholinergic release or subthreshold oscillation functions to control the cellular processes responsible for memory formation and spatial navigation.
Ben Strowbridge (Advisor)
Stephen Jones (Committee Chair)
Corey Smith (Committee Member)
Witold Surewicz (Committee Member)
Christopher Ford (Committee Member)
David Friel (Committee Member)
140 p.
Animals; Biology; Biophysics; Health Sciences; Neurobiology; Neurosciences; Physiology
In vivo recordings; whole cell patch clamp; hippocampus; mouse; dentate gyrus; granule cell; mossy cell; interneuron; acetylcholine; head-fixed awake recordings; persistent firing; plateau potential; current clamp; subthreshold oscillations; alpha

Recommended Citations

Citations

  • Anderson, R. W. (2015). Subthreshold Oscillations and Persistent Activity Modulate Spike Output in the Rodent Dentate Gyrus [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1415229722

    APA Style (7th edition)

  • Anderson, Ross. Subthreshold Oscillations and Persistent Activity Modulate Spike Output in the Rodent Dentate Gyrus. 2015. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1415229722.

    MLA Style (8th edition)

  • Anderson, Ross. "Subthreshold Oscillations and Persistent Activity Modulate Spike Output in the Rodent Dentate Gyrus." Doctoral dissertation, Case Western Reserve University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1415229722

    Chicago Manual of Style (17th edition)

case1415229722
428
© 2014, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.