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Investigating Hemodynamics of the Developing Embryonic Heart using Optical Coherence Tomography

Peterson, Lindsy Marie
http://rave.ohiolink.edu/etdc/view?acc_num=case1428056825

Abstract Details

2015, Doctor of Philosophy, Case Western Reserve University, Biomedical Engineering.
Blood flow is a critical factor that regulates developmental programs during cardiogenesis. During early embryonic development, deviations from the normal blood flow pattern have been shown to lead to congenital heart defects including septal defects and outflow tract anomalies. To better understand the role flow and the resulting hemodynamic forces play during cardiovascular development precise tools are needed to rapidly calculate and monitor these forces. Optical coherence tomography (OCT) is a noninvasive imaging modality that is well suited for imaging the developing heart due to its high spatial and temporal resolution. OCT is also capable of analyzing various cardiac functions by measuring the blood flow through the developing heart via the Doppler effect. Here we present several techniques we have developed that use structural and Doppler OCT to monitor and measure hemodynamic parameters in the early embryonic heart. First, we generated 4-D (3-D volumes over time) shear stress maps from Doppler OCT data sets. These maps enabled comparisons of shear stress from the inner curvature versus the outer curvature at different regions of the looping heart tube over the duration of a heartbeat. We also developed an orientation independent technique for measuring the absolute blood flow in a vessel from individual cross sectional images. This technique utilizes a dual angle delay encoding technique to obtain instantaneous pulsatile blood flow measurements irrespective of the vessel orientation. We used this technique to image the aortic arches in control and ethanol exposed embryos. The aortic arches undergo a significant morphogenesis from a symmetrical system of paired vessels to an asymmetrical structure and hemodynamics is thought to play a critical role in this transformation. Blood flow and shear stress were both calculated as well as the cross sectional area of the pharyngeal tissue surrounding the aortic arch vessel. Finally, we developed a technique for measuring the contractile wave velocity in different regions of the heart tube. These values were then compared with conduction velocities obtained from optical mapping. Here we have developed and demonstrated a range of novel techniques for measuring cardiac function in the developing embryonic heart. These techniques may prove to be powerful tools for future investigations of the role of blood flow and the resulting hemodynamic forces play in congenital heart defects.
Andrew Rollins, PhD (Committee Chair)
Michiko Watanabe, PhD (Committee Member)
David Wilson, PhD (Committee Member)
Kenneth Singer, PhD (Committee Member)
150 p.
Biomedical Engineering; Developmental Biology
optical coherence tomography; doppler; hemodynamics; embryonic heart development; shear stress; absolute flow; aortic arch;

Recommended Citations

Citations

  • Peterson, L. M. (2015). Investigating Hemodynamics of the Developing Embryonic Heart using Optical Coherence Tomography [Doctoral dissertation, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1428056825

    APA Style (7th edition)

  • Peterson, Lindsy. Investigating Hemodynamics of the Developing Embryonic Heart using Optical Coherence Tomography. 2015. Case Western Reserve University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1428056825.

    MLA Style (8th edition)

  • Peterson, Lindsy. "Investigating Hemodynamics of the Developing Embryonic Heart using Optical Coherence Tomography." Doctoral dissertation, Case Western Reserve University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1428056825

    Chicago Manual of Style (17th edition)

case1428056825
584
© 2015, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.