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Thesis_Yehe Liu.pdf (2.39 MB)
ETD Abstract Container
Abstract Header
A NOVEL METHOD TO EXTRACT TYPE-I COLLAGEN FIBRILS FROM MAMMALIAN TENDONS
Author Info
Liu, Yehe
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=case1433206202
Abstract Details
Year and Degree
, Master of Sciences (Engineering), Case Western Reserve University, Biomedical Engineering.
Abstract
A novel method to extract collagen fibrils from mammalian tendons solution has been developed. Tissue is cut on the surface and soaked in a trypsin based extraction medium. The mixture is shaken by hand, and the tissue becomes swollen in 5 minutes. The swollen tissue is washed and soaked in an aqueous solution, and separated by pulling using two pairs of tweezers. Collagen fibrils are released into the aqueous solution. Inspected using dark-field microscopy, electron microscopy, turbidity measurement and electrophoresis, it was proven that this method would not cause significant morphological damage to the extracted collagen fibrils. This method is particularly useful in preparing collagen fibrils samples for related mechanical tests, from mammalian tendons and other types of collagenous tissue. It is also useful in producing collagen fibrils as materials for other biomedical applications.
Committee
Steven Eppell (Advisor)
Vincent Hascall (Committee Member)
Jeffrey Capadona (Committee Member)
Pages
146 p.
Subject Headings
Biomedical Engineering
;
Biomedical Research
Keywords
Collagen
;
Fibril
;
Tendon
;
Mechanical Measurements
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Citations
Liu, Y. (2015).
A NOVEL METHOD TO EXTRACT TYPE-I COLLAGEN FIBRILS FROM MAMMALIAN TENDONS
[Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1433206202
APA Style (7th edition)
Liu, Yehe.
A NOVEL METHOD TO EXTRACT TYPE-I COLLAGEN FIBRILS FROM MAMMALIAN TENDONS.
2015. Case Western Reserve University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=case1433206202.
MLA Style (8th edition)
Liu, Yehe. "A NOVEL METHOD TO EXTRACT TYPE-I COLLAGEN FIBRILS FROM MAMMALIAN TENDONS." Master's thesis, Case Western Reserve University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1433206202
Chicago Manual of Style (17th edition)
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Document number:
case1433206202
Download Count:
4,921
Copyright Info
© 2015, some rights reserved.
A NOVEL METHOD TO EXTRACT TYPE-I COLLAGEN FIBRILS FROM MAMMALIAN TENDONS by Yehe Liu is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.