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LIS-1 INHERITANCE IN CROSSES AMONGST FLAX VARIETIES

Piechowski, Jennifer M
http://rave.ohiolink.edu/etdc/view?acc_num=case1433248469

Abstract Details

2015, Master of Sciences, Case Western Reserve University, Biology.
Linum Insertion Sequence-1 (LIS-1) is a 5.7kb region of DNA that is found in various flax (Linum usitatissimum) varieties in response to environmental stress, such as when grown under low nutrient inducing conditions. It was determined that flax can be successfully transformed via a novel floral dip method in which exogenous LIS-1 was inserted into the genome of two flax varieties, PL (Stormont cirrus) and Bethune, neither of which have endogenous LIS-1. Once inserted, it was found that LIS-1 was not stably inherited in the offspring of PL, when grown under inducing conditions, nor Bethune regardless of growth conditions. Due to this instability of exogenous LIS-1, the stability of endogenous LIS-1 in the F2 plants from crosses between the flax varieties PL and Stormont motley were studied. Here, it was observed that endogenous LIS-1 is stably inherited in a normal Mendelian fashion with an approximate 1:2:1 segregation ratio.
Christopher Cullis, PhD (Advisor)
Emmitt Jolly, PhD (Committee Member)
David Burke, PhD (Committee Member)
57 p.
Biology; Genetics; Molecular Biology

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Citations

  • Piechowski, J. M. (2015). LIS-1 INHERITANCE IN CROSSES AMONGST FLAX VARIETIES [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1433248469

    APA Style (7th edition)

  • Piechowski, Jennifer. LIS-1 INHERITANCE IN CROSSES AMONGST FLAX VARIETIES. 2015. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1433248469.

    MLA Style (8th edition)

  • Piechowski, Jennifer. "LIS-1 INHERITANCE IN CROSSES AMONGST FLAX VARIETIES." Master's thesis, Case Western Reserve University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1433248469

    Chicago Manual of Style (17th edition)

case1433248469
467
© 2015, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.