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IN SITU FORMING PHOTODEGRADABLE HYDROGEL FOR CONTROLLED DELIVERY OF siRNA

Zheng, Zijie
http://rave.ohiolink.edu/etdc/view?acc_num=case1434560306

Abstract Details

2015, Master of Sciences, Case Western Reserve University, Biomedical Engineering.
Cells adapt themselves to the dynamic extracellular environment by responding to numerous signal stimuli. Strategies for engineering stimulus-responsive biomaterials as drug delivery vehicles may mimic this responsiveness and allow for spatiotemporal control over drug delivery. Short interfering RNA (siRNA) can inhibit specific gene expression by cleaving complementary mRNA molecules, which may be useful when presented in a stimulus-responsive manner. Here, a photodegradable hydrogel system was developed for siRNA delivery, and the release of siRNA complexed with poly(ethyleneimine) (siRNA/PEI) from photodegradable hydrogels was tailored by the trigger of UV exposure. Photodegradable poly (ethylene glycol)-based hydrogels containing siRNA/PEI complexes have been successfully fabricated, and light-triggered release of siRNA and gene-silencing abilities of released siRNA in cells cultured in monolayer has been shown using green fluorescent protein as a reporter. Future work can extend this platform technology to temporally control biologically relevant processes in an “on-demand” manner.
Eben Alsberg (Advisor)
Zheng-Rong Lu (Committee Member)
Nicole Steinmetz (Committee Member)
74 p.
Biomedical Engineering
siRNA, photodegradable, hydrogel, controlled release

Recommended Citations

Citations

  • Zheng, Z. (2015). IN SITU FORMING PHOTODEGRADABLE HYDROGEL FOR CONTROLLED DELIVERY OF siRNA [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1434560306

    APA Style (7th edition)

  • Zheng, Zijie. IN SITU FORMING PHOTODEGRADABLE HYDROGEL FOR CONTROLLED DELIVERY OF siRNA . 2015. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1434560306.

    MLA Style (8th edition)

  • Zheng, Zijie. "IN SITU FORMING PHOTODEGRADABLE HYDROGEL FOR CONTROLLED DELIVERY OF siRNA ." Master's thesis, Case Western Reserve University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1434560306

    Chicago Manual of Style (17th edition)

case1434560306
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© 2015, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.