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MULTICEULLULAR TUMOR HEMI-SPHEROID: A NOVEL IN VITRO 3D MODEL PLATFORM FOR ACCELERATED DRUG DEVELOPMENT

Kim, Kihwan
http://orcid.org/0000-0002-5003-4378
http://rave.ohiolink.edu/etdc/view?acc_num=case1481900120946458

Abstract Details

2017, Master of Sciences, Case Western Reserve University, Biomedical Engineering.
Multicellular tumor spheroid (MCTS) is currently the most accepted 3D in vitro tumor model. MCTS can mimic in vivo avascular tumor and often used in drug development studies before the in vivo animal experiment. Despite of its recognized values, MCTS is still not widely implemented in drug development pipeline. This is because culturing MCTS to an appropriate size (above 300µm) is often difficult and assays to analyze drug penetration in MCTS relies on the information of lumped cell death and biomarkers after exposure to drugs. We have developed a novel 3D in vitro model called Multicellular tumor hemi-spheroid (MTHS). MTHS shares same nutrient and oxygen distribution with MCTS due to similarity in the geometric shape. Also the flat equatorial surface of the MTHS can act as a window to its inner region. With this key feature cell viability and drug distribution can be mapped and oxygen distribution quantified non-invasively. This thesis describes the process of MTHS culture using an agarose scaffold approach, characterization of MTHS and viability and drug penetration assays using MTHS.
Miklos Gratzl (Committee Chair)
David Wilson (Committee Member)
Agata Exner (Committee Member)
96 p.
Biomedical Engineering; Biomedical Research

Recommended Citations

Citations

  • Kim, K. (2017). MULTICEULLULAR TUMOR HEMI-SPHEROID: A NOVEL IN VITRO 3D MODEL PLATFORM FOR ACCELERATED DRUG DEVELOPMENT [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1481900120946458

    APA Style (7th edition)

  • Kim, Kihwan. MULTICEULLULAR TUMOR HEMI-SPHEROID: A NOVEL IN VITRO 3D MODEL PLATFORM FOR ACCELERATED DRUG DEVELOPMENT. 2017. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1481900120946458.

    MLA Style (8th edition)

  • Kim, Kihwan. "MULTICEULLULAR TUMOR HEMI-SPHEROID: A NOVEL IN VITRO 3D MODEL PLATFORM FOR ACCELERATED DRUG DEVELOPMENT." Master's thesis, Case Western Reserve University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1481900120946458

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.