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Daniel Varghai Thesis.pdf (70.59 MB)

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Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration

Varghai, Daniel
http://orcid.org/0000-0003-1454-7931
http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966

Abstract Details

2017, Master of Engineering, Case Western Reserve University, Biomedical Engineering.
Lower-limb fracture exhibits a limited capacity to heal critically sized defects and biomimetic tissue engineering is a promising approach for addressing this clinical need. Here, scaffold-free tubular mesenchymal condensations featuring temporally controlled TGF-Β1 and BMP-2 morphogen release from incorporated microparticles were engineered to form self-assembled rings and tubes. In vitro culture, subcutaneous, and femoral defect implantation was performed to establish bone forming capacity with the treatment groups: 1) TGF-Β1, 2) BMP-2, or 3) BMP-2+TGF-Β1. Bone formation was evaluated by biochemical, μCT, and histological analyses. Dual-delivery enhanced bone volume versus single morphogens at 6wks, and histology revealed bone within tubular geometries with enhanced cartilage, mimetic of endochondral ossification. Overall: 1) geometry of scaffold-free mesenchymal condensations guided 3D ossification, 2) BMP-2+TGF-Β1 presentation augmented ectopic bone formation, and 3) tubular architecture promoted femoral defect bridging over random organization.
Eben Alsberg, Dr. (Advisor)
Steven Eppell, Dr. (Committee Member)
Ozan Akkus, Dr. (Committee Member)
105 p.
Biology; Biomedical Engineering; Biomedical Research; Cellular Biology; Engineering; Health Sciences; Histology; Medical Imaging; Microbiology; Morphology
Growth factors; TGF-B1; BMP-2; controlled delivery; microparticles; chondrogenesis; osteogenesis; mold; self-assembly; regenerative medicine;

Recommended Citations

Citations

  • Varghai, D. (2017). Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966

    APA Style (7th edition)

  • Varghai, Daniel. Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration. 2017. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966.

    MLA Style (8th edition)

  • Varghai, Daniel. "Tubular Tissue Engineered Scaffold-Free High-Cell-Density Mesenchymal Condensations For Femoral Defect Regeneration." Master's thesis, Case Western Reserve University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1497222797338966

    Chicago Manual of Style (17th edition)

case1497222797338966
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© 2017, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.