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Final report- v7.pdf (1.05 MB)

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Development of a Z-Stack Projection Imaging Protocol for a Nerve Allograft

Selvam, Selvaanish
http://orcid.org/0000-0002-6707-7614
http://rave.ohiolink.edu/etdc/view?acc_num=case1531235036419173

Abstract Details

2018, Master of Sciences (Engineering), Case Western Reserve University, Biomedical Engineering.
Peripheral nerve injuries have traditionally been treated with a variety of different surgical procedures, but the use of allografts for these injuries remain to be a largely unexplored concept. As a result, there has yet to be a successful protocol created for imaging cells on the surface of nerve allografts. In this thesis, I developed a z-stack projection imaging protocol for nerve allografts and demonstrated the ability to take clear and focused images of cell retention and proliferation on the surface of the allograft. I also optimized the preparation of the nerve graft to improve cell and connective progenitor cell (CTP) retention rates.
George Muschler, MD (Committee Chair)
Eben Alsberg, PhD (Advisor)
Robert Kirsch, PhD (Committee Member)
Cynthia Boehm (Committee Member)
56 p.
Biomedical Engineering; Cellular Biology; Optics
Imaging, Z-stack, projection image, Z-stack projection image, nerve allograft, peripheral nerve injuries, cell culture, dapi imaging, connective tissue progenitor, CTP, fluorescent imaging, depth of field, cell retention, loading efficiency, BMC

Recommended Citations

Citations

  • Selvam, S. (2018). Development of a Z-Stack Projection Imaging Protocol for a Nerve Allograft [Master's thesis, Case Western Reserve University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=case1531235036419173

    APA Style (7th edition)

  • Selvam, Selvaanish. Development of a Z-Stack Projection Imaging Protocol for a Nerve Allograft . 2018. Case Western Reserve University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=case1531235036419173.

    MLA Style (8th edition)

  • Selvam, Selvaanish. "Development of a Z-Stack Projection Imaging Protocol for a Nerve Allograft ." Master's thesis, Case Western Reserve University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1531235036419173

    Chicago Manual of Style (17th edition)

case1531235036419173
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© 2018, all rights reserved.
This open access ETD is published by Case Western Reserve University School of Graduate Studies and OhioLINK.