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Molecular Mechanism of Incorporation of Factor Va into Prothrombinase

Barhoover, Melissa
http://rave.ohiolink.edu/etdc/view?acc_num=csu1197570167

Abstract Details

2007, Doctor of Philosophy in Clinical-Bioanalytical Chemistry, Cleveland State University, College of Science.
The mainstay of the blood coagulation cascade is the formation of the fibrin clot, catalyzed by the serine protease, thrombin. The prothrombinase complex catalyzes the activation of prothrombin to thrombin and, is composed of the enzyme, factor Xa, and the protein cofactor, factor Va, in the presence of divalent metal ions associated on a membrane surface. The enzyme, factor Xa, alone can activate prothrombin by two sequential proteolytic cleavages at Arg271 and Arg320 resulting in the intermediates, Fragment 1.2 and Prethrombin 2. The overall rate of this reaction is not compatible with survival. On the other hand, the incorporation of an excess of the cofactor, factor Va, into prothrombinase reverses the order of the proteolytic cleavages and increases the catalytic activity of factor Xa by 5 orders of magnitude, making this reaction compatible with survival. The goal of this research is to identify the precise amino acids of the central portion of the factor Va heavy chain involved in its incorporation into the prothrombinase complex and the cofactor function it exerts on the catalytic efficiency of prothrombin activation.
Dr. Michael Kalafatis (Committee Chair)
Dr. Lily Ng (Other)
Dr. Amin Zhou (Other)
157 p.
Factor V; Blood Coagulation; Prothrombinase; APC; Thrombin; Factor V Leiden

Recommended Citations

Citations

  • Barhoover, M. (2007). Molecular Mechanism of Incorporation of Factor Va into Prothrombinase [Doctoral dissertation, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1197570167

    APA Style (7th edition)

  • Barhoover, Melissa. Molecular Mechanism of Incorporation of Factor Va into Prothrombinase. 2007. Cleveland State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1197570167.

    MLA Style (8th edition)

  • Barhoover, Melissa. "Molecular Mechanism of Incorporation of Factor Va into Prothrombinase." Doctoral dissertation, Cleveland State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=csu1197570167

    Chicago Manual of Style (17th edition)

csu1197570167
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© 2007, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.