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Fabricating New Miniaturized Biosensors for the Detection of DNA Damage and DNA Mismatches

Perera, Naullage I.
http://rave.ohiolink.edu/etdc/view?acc_num=csu1244139647

Abstract Details

2009, Doctor of Philosophy in Clinical-Bioanalytical Chemistry, Cleveland State University, College of Science.
A large number of genetic diseases and genetic disorders are simply caused by base alterations in the genome. Therefore, developing efficient and cost effective techniques for routine detection of these alterations is of great importance. Different methods involving gel electrophoresis and Polymerase Chain Reaction have been widely employed, but majority of these methods are costly, time consuming, and lack throughput, creating a fundamental gap between the current state-of-the-art and desired characteristics of low-cost, high-speed, simplicity, versatility, and potential for miniaturization. In this study, we attempt to bridge this gap by developing new sensing platforms to detect DNA base mismatches and DNA damage with higher throughput, better ease-of-use, and with the potential to be miniaturized for greater portability. Two electrochemical mismatch detection sensing platforms were developed. One uses the electrochemical reduction of trans-4-cinnamic acid diazonium tetrafluoroborate. The other takes advantage of the natural ability of MutS protein for single base mismatch recognition. Also, two DNA damage detection assays were developed and the first approach uses Atomic Force Microscopy to monitor minor DNA damage by labeling damaged sites with a biomarker. This site-specific biolabeling was achieved through well-established biotin-streptavidin chemistry. In the second approach, a new layer-by-layer biomolecular immobilization method was introduced and used to detect DNA chemical damage using electrochemical techniques.
Mekki Bayachou, PhD (Committee Chair)
Lily Ng, PhD (Committee Member)
Robert Wei, PhD (Committee Member)
John Masnovi, PhD (Committee Member)
Crystal Weyman, PhD (Committee Member)
148 p.
Analytical Chemistry
DNA mismatch detection; AFM; DNA damage detection; electrochemical sensors

Recommended Citations

Citations

  • Perera, N. I. (2009). Fabricating New Miniaturized Biosensors for the Detection of DNA Damage and DNA Mismatches [Doctoral dissertation, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1244139647

    APA Style (7th edition)

  • Perera, Naullage. Fabricating New Miniaturized Biosensors for the Detection of DNA Damage and DNA Mismatches. 2009. Cleveland State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1244139647.

    MLA Style (8th edition)

  • Perera, Naullage. "Fabricating New Miniaturized Biosensors for the Detection of DNA Damage and DNA Mismatches." Doctoral dissertation, Cleveland State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1244139647

    Chicago Manual of Style (17th edition)

csu1244139647
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© 2009, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.