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Regulation of Apoptosis by the Muscle Regulatory Transcription Factor MyoD

Harford, Terri J.
http://rave.ohiolink.edu/etdc/view?acc_num=csu1265812933

Abstract Details

2009, Doctor of Philosophy in Regulatory Biology, Cleveland State University, College of Science.
We have previously reported that the level of MyoD expression correlates with the level of apoptosis that occurs in a subpopulation of skeletal myoblasts induced to differentiate by serum withdrawal. Herein we document that MyoD expression dictates the apoptotic threshold in myoblasts and fibroblasts in response to a variety of apoptotic stimuli. Specifically, re-expression of MyoD inskeletal myoblasts rendered defective for both differentiation and apoptosis by the expression of oncogenic Ras restores their ability to undergo both differentiation and apoptosis in response to serum withdrawal. Further, using a fibroblast cell line expressing an estrogen receptor:MyoD fusion protein, we have determined that addition of estrogen sensitizes these fibroblasts to apoptosis induced by serum withdrawal, or by treatment with etoposide or thapsigargin. RNAi mediated silencing of MyoD in either 23A2 or C2C12 myoblasts renders these cells resistant to apoptosis induced by serum withdrawal, or by treatment with etoposide or thapsigargin. Finally, MyoD mediated regulation of the apoptotic response to these various stimuli correlates with the level of induction of the pro-apoptotic Bcl-2 family member PUMA.
Crystal Weyman, PhD (Advisor)
Anton Komar, PhD (Committee Member)
Barsanjit Mazumder, PhD (Committee Member)
Martha Cathcart, PhD (Committee Member)
Alexandru Almasan (Other)
Andrew Resnick, PhD (Other)
120 p.
Cellular Biology; Molecular Biology
MyoD; PUMA; apoptosis

Recommended Citations

Citations

  • Harford, T. J. (2009). Regulation of Apoptosis by the Muscle Regulatory Transcription Factor MyoD [Doctoral dissertation, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1265812933

    APA Style (7th edition)

  • Harford, Terri. Regulation of Apoptosis by the Muscle Regulatory Transcription Factor MyoD. 2009. Cleveland State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1265812933.

    MLA Style (8th edition)

  • Harford, Terri. "Regulation of Apoptosis by the Muscle Regulatory Transcription Factor MyoD." Doctoral dissertation, Cleveland State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1265812933

    Chicago Manual of Style (17th edition)

csu1265812933
641
© 2009, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.