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Targets of Hsa-miR-488* In Human Prostate Carcinoma Cells

Slaibi, Jinani Elias
http://rave.ohiolink.edu/etdc/view?acc_num=csu1273843449

Abstract Details

2010, Master of Science in Biology, Cleveland State University, College of Liberal Arts and Social Sciences.
Prostate cancer (PCa) is one of the most prevalent forms of cancer among men in America and is second only to lung cancer as a cause of cancer-related deaths in men. Recent epidemiological study shows that one in every six men over the age of forty five is at risk of PCa. Androgen receptor (AR) plays a causative role in the development of PCa. Hormonal blockade therapy which inhibits the expression of AR eventually fails and disease progresses to fatal androgen-refractory stage from androgen-dependent stage. Therefore, novel molecular approaches which can target and block the expression of AR are required. We propose that microRNAs (miRNA) that function as negative gene regulators have potential as PCa therapeutics. Using bioinformatics methods, we have identified that human miRNA hsa-miR-488* has potential to modulate AR expression. In the present study, we have validated the target site in AR 3’UTR and established that AR is a target of Hsa-miR-488*. Our data show that the ectopically expressed hsa-miR-488* as well as the synthetic miRNA mimic can suppress the expression of luciferase activity in chimeric plasmid harboring AR3’UTR with dose dependent effects. In addition, miR-488* negatively regulated the expression of endogenous androgen receptor in PCa cells LNCaP. Thus hsa-miR-488* that function as negative gene regulators has potential as PCa therapeutics.
Girish Shukla, PhD (Advisor)
Crystal Weyman, PhD (Committee Member)
Barsanjit Mazumder, PhD (Committee Member)
75 p.
Bioinformatics; Biology; Biomedical Research
miR-488; miRNA; UTR; Androgen; luciferase; AR; target site

Recommended Citations

Citations

  • Slaibi, J. E. (2010). Targets of Hsa-miR-488* In Human Prostate Carcinoma Cells [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1273843449

    APA Style (7th edition)

  • Slaibi, Jinani. Targets of Hsa-miR-488* In Human Prostate Carcinoma Cells. 2010. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1273843449.

    MLA Style (8th edition)

  • Slaibi, Jinani. "Targets of Hsa-miR-488* In Human Prostate Carcinoma Cells." Master's thesis, Cleveland State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=csu1273843449

    Chicago Manual of Style (17th edition)

csu1273843449
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© 2010, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.