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Two Approaches for Cell Retention in Perfusion Culture Systems

Wang, Zhaowei
http://rave.ohiolink.edu/etdc/view?acc_num=csu1274106674

Abstract Details

2009, Doctor of Engineering, Cleveland State University, Fenn College of Engineering.
A lack of efficient, economical, and reliable cell retention devices has limited the application of perfusion culture systems in the biopharmaceutical industry. Two types of cell retention devices were developed in this work for long-term perfusion culture systems: a modification of an inclined gravity settler and a variation of an ultrasonic filter. Both bench-top and large-scale tests showed that the gravity settler can effectively retain viable cells and preferentially remove nonviable cells in perfusion culture systems. The viable cell retention rate can be maintained well above 90% during long-term perfusion culture period while the nonviable cell retention rate is 20-30% lower than that of viable cells. The design of this settler enables its manufacture as a single-use device to be used in conjunction with disposable bioreactors. An apparatus for measuring the settling velocities of both the viable and nonviable cells, which is both simple and inexpensive to use, was developed in order to predict optimal operation parameters of the gravity settler. The gravity settler was also effective in an algae dewatering process. The ultrasonic filter has a cell retention capacity 21-fold greater than that of the gravity settler for the same working surface area. Due to its unique design, this ultrasonic filter can operate continuously, as opposed to the currently available ultrasonic filters that need on-off intervals for removal of retained cells.
Joanne Belovich, PhD (Advisor)
Cahir McDevitt, PhD (Committee Member)
George Chatzimavroudis, PhD (Committee Member)
Donald Feke, PhD (Committee Member)
Crystal Weyman, PhD (Committee Member)
167 p.
Biomedical Research; Chemical Engineering
Cell culture; Perfufsion culture; Cell retention; Gravity settler; Settling velocity; Algae concentration; Ultrasonic filter; Particle separation

Recommended Citations

Citations

  • Wang, Z. (2009). Two Approaches for Cell Retention in Perfusion Culture Systems [Doctoral dissertation, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1274106674

    APA Style (7th edition)

  • Wang, Zhaowei. Two Approaches for Cell Retention in Perfusion Culture Systems. 2009. Cleveland State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1274106674.

    MLA Style (8th edition)

  • Wang, Zhaowei. "Two Approaches for Cell Retention in Perfusion Culture Systems." Doctoral dissertation, Cleveland State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1274106674

    Chicago Manual of Style (17th edition)

csu1274106674
2,264
© 2009, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.