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One-Dimensional Radial Diffusion of Small Molecules (376 Da) in Bone Tissue

Farrell, Kurt W.
http://rave.ohiolink.edu/etdc/view?acc_num=csu1328131831

Abstract Details

2011, Master of Science in Biomedical Engineering, Cleveland State University, Fenn College of Engineering.
The flow of nutrients through any biological tissue is important to maintain homeostasis. If the transport process is understood, medical research teams can better design medications, prosthetic implants, and tissue scaffolds. Additionally, transport rates help physicians to better understand disease states and wound healing, including minor injuries such as breaks and sprains, which will aid in better diagnoses. We developed a novel method that measures the rate of diffusion in vitro, of fluorescein sodium salt. Samples were incubated at 37°C in a 5% CO2 atmosphere for various periods of time. Samples were sliced and analyzed using Image-Pro Plus and MATLAB to obtain concentration profiles. The diffusivity was estimated from the data using the model equation for one-dimensional transport in a finite medium. We found that radial diffusivity in canine bone in 1-dimension was 1.27 x 10-7±1.96 x 10-8 cm2/s. As a point of reference, the diffusivity of fluorescein sodium salt in PBS is 2.7 x 10-6 cm2/s. Given the average distance between a Haversian canal and an osteon radius is 250 μm, our data shows it would take approximately 20 minutes for a nutrient of a weight of 376 Da to travel between the two locations. This indicates that the diffusion time of key nutrients, such as vitamin D, with molecular weight of 384 Da, would be about 20 minutes.
Joanne Belovich, PhD (Committee Chair)
Surendra Tewari, PhD (Committee Member)
Ronald Midura, PhD (Committee Member)
138 p.
Biology; Biomechanics; Biomedical Engineering; Biomedical Research; Biophysics; Fluid Dynamics; Health Sciences; Medicine
bone tissue; diffusion; radial; tissue engineering; fluorescence microscopy; biomedical engineering; osseous tissue; fluid dynamics; transport phenomena; unsteady state

Recommended Citations

Citations

  • Farrell, K. W. (2011). One-Dimensional Radial Diffusion of Small Molecules (376 Da) in Bone Tissue [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1328131831

    APA Style (7th edition)

  • Farrell, Kurt. One-Dimensional Radial Diffusion of Small Molecules (376 Da) in Bone Tissue. 2011. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1328131831.

    MLA Style (8th edition)

  • Farrell, Kurt. "One-Dimensional Radial Diffusion of Small Molecules (376 Da) in Bone Tissue." Master's thesis, Cleveland State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=csu1328131831

    Chicago Manual of Style (17th edition)

csu1328131831
292
© 2011, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.