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merged_final thesis-5-22-2013.pdf (1.82 MB)

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LIPID PRODUCTION BY SCENEDESMUS DIMORPHUS COMPARISON: OF EXTRACTION TECHNIQUES AND EFFECT OF SODIUM NITRATE CONCENTRATION

Kanani, Bahareh
http://rave.ohiolink.edu/etdc/view?acc_num=csu1370519484

Abstract Details

2013, Master of Science in Chemical Engineering, Cleveland State University, Fenn College of Engineering.
Exhaustion of fossil fuels, negative effects of excess carbon dioxide in the atmosphere and high gas prices have all made it very crucial to find alternative and renewable sources of energy. Scientists are studying biological and physiological potential of microalgae as an alternative source for biofuel production. Microalgae have shown to be more economical among other crops including corn, sunflower and so on. They are also able to reduce industrial carbon dioxide by consuming it through the growth process and can be grown almost everywhere. Microalgae also have high growth rate which means higher production in a shorter time. They need less land compare to other crop for the same amount of lipid or oil. Among more than 100 species of microalgae, one with great lipid potential is Scenedesmus dimorphus. In this study we developed and evaluated five different permeabilization methods to determine the best lipid extraction method to produce more lipids from S. dimorphus biomass. The methods which were used in this research include wet-pellet, freeze-dry, sonication, grinding and freeze-thaw. Our results show that the most promising method is the grinding method using mortar and pestle, followed by solvent extraction in hexane/isopropanol (3:2, v/v). Different types of batch experiments were performed to determine growth parameters such as growth rate (µ), yield (Yx/s) and lipid content (Y). The effect of sodium nitrate concentration on growth rate and lipid content was also studied using a fed-batch experiment with constant concentration of sodium nitrate. This could be resulted that the maximum content of lipid occurred in the medium with 0.00 g NaNO3/L (flask 4). Data shows that flask 3 (0.07 g NaNO3/L) has the highest growth rate among the flasks (µ=0.47 day -1). The results are helpful to better understand the effect of nutrients on lipid content.
Joanne M. Belovich, PhD (Committee Chair)
Jorge E. Gatica, PhD (Committee Member)
Chandrasekhar Kothapalli, PhD (Committee Member)
Dhananjai B. Shah, PhD (Committee Member)
98 p.
Chemical Engineering

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Citations

  • Kanani, B. (2013). LIPID PRODUCTION BY SCENEDESMUS DIMORPHUS COMPARISON: OF EXTRACTION TECHNIQUES AND EFFECT OF SODIUM NITRATE CONCENTRATION [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1370519484

    APA Style (7th edition)

  • Kanani, Bahareh. LIPID PRODUCTION BY SCENEDESMUS DIMORPHUS COMPARISON: OF EXTRACTION TECHNIQUES AND EFFECT OF SODIUM NITRATE CONCENTRATION. 2013. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1370519484.

    MLA Style (8th edition)

  • Kanani, Bahareh. "LIPID PRODUCTION BY SCENEDESMUS DIMORPHUS COMPARISON: OF EXTRACTION TECHNIQUES AND EFFECT OF SODIUM NITRATE CONCENTRATION." Master's thesis, Cleveland State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1370519484

    Chicago Manual of Style (17th edition)

csu1370519484
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This open access ETD is published by Cleveland State University and OhioLINK.