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SYNTHESIS AND CHARACTERIZATION OF pH-RESPONSIVE ELASTIN-LIKE POLYPEPTIDES WITH DIFFERENT CONFIGURATIONS

Tang, Mingjie
http://rave.ohiolink.edu/etdc/view?acc_num=csu1376784321

Abstract Details

2013, Master of Science in Chemical Engineering, Cleveland State University, Fenn College of Engineering.
Elastin-like polypeptides (ELP) are environmentally responsive polymers that exhibit phase transition behavior in response to various stimuli such as temperature, pH and irradiation. In this work, we focused on pH-responsive ELPs. It has been shown that pH value, configurations of ELPs, ELP concentration, and salt concentration can impact the phase transition behavior of pH-responsive ELPs. Quantitative models have been developed to engineer various linear ELPs, but there has not been any detailed study for engineering ionizable ELP with non-linear configurations. In this study, we designed, synthesized, and characterized pH-responsive ELPs with two different configurations. One is linear ELP, (GVGVPGEGVPGVGVP)12, and the other one is a three-armed star ELP, (GVGVPGEGVPGVGVP)12-foldon. Three ELP-foldon chains fold as a trimer in solutions. These constructs were synthesized by molecular biology techniques and characterized by different methods. A modified model based on previous studies was used to describe the pH-dependent reversible phase transition in response to their solution environment. The model was validated with pH-responsive ELPs with different configurations, i.e., linear and trimer. The phase transition of both ELPs fit this model across a range of pH. These results demonstrate that we are able to rationally design responsive polypeptides with different configurations whose transition can be triggered at a specified pH.
Nolan Holland, PhD (Advisor)
Chandra Kothapalli, PhD (Committee Member)
Surendra Tewari , PhD (Committee Member)
Aimin Zhou, PhD (Committee Member)
Biomedical Engineering; Chemical Engineering
pH-responsive, ELPs

Recommended Citations

Citations

  • Tang, M. (2013). SYNTHESIS AND CHARACTERIZATION OF pH-RESPONSIVE ELASTIN-LIKE POLYPEPTIDES WITH DIFFERENT CONFIGURATIONS [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1376784321

    APA Style (7th edition)

  • Tang, Mingjie. SYNTHESIS AND CHARACTERIZATION OF pH-RESPONSIVE ELASTIN-LIKE POLYPEPTIDES WITH DIFFERENT CONFIGURATIONS. 2013. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1376784321.

    MLA Style (8th edition)

  • Tang, Mingjie. "SYNTHESIS AND CHARACTERIZATION OF pH-RESPONSIVE ELASTIN-LIKE POLYPEPTIDES WITH DIFFERENT CONFIGURATIONS." Master's thesis, Cleveland State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1376784321

    Chicago Manual of Style (17th edition)

csu1376784321
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© 2013, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.