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Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model

Jensen, Rebecca Leah
http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419

Abstract Details

2013, Master of Science in Chemical Engineering, Cleveland State University, Fenn College of Engineering.
Under this research, live cell imaging of osteoblast-like marrow stromal cells has been carried out on polished and nanotextured (NaOH-etched) medical-grade titanium alloy (Ti-6Al-4V) surfaces to examine cellular adhesion and migration. The purpose of this research was to: 1) Build and assemble suitable hardware and software to conduct live cell imaging in a micro-incubator over an extended period of time. 2) Monitor and record live osteoblast-like marrow stromal cells on polished and NaOH-etched titanium alloy surfaces from cell inoculation to about one week of culture. 3) Measure location, area and perimeter of individual cells as a function of time, and examine if, as compared / contrasted with the polished titanium surface, that the NaOH-etched titanium surface promotes adhesion and migration of cells. This was achieved by describing the mobility, morphology and overall behavior of osteoblast-like marrow stromal cells. During the cell growth cycles, data generated from image analysis included the cells’ center of mass (X,Y), their area, perimeter and shape as a function of incubation time. From the change in center of mass after each 15-minute interval, the real time speed of the cells was obtained. Major observations to support comparison studies between the surfaces determined that compared with polished titanium, NaOH-etched titanium promotes cellular filopodia growth, thus, promotes attachment. Filopodia provide cellular anchoring support and when prevalent, make cells more angular in shape. The median aspect ratio (length / width) of cells was found to be 1.38 on polished and 2.36 on NaOH-etched titanium. This, in addition to lower mean circularity shape factor values of 0.26 ± 0.03 on polished and 0.11 ± 0.01 on NaOH-etched titanium imply that the nanotextured surface promotes growth of cells more anchored to the substrate. This is also confirmed by increased perimeters of cells found on the NaOH-etched surface (950.92 ± 84.88 μm) compared with perimeter lengths of cells on the polished surface (668.55 ± 22.55 μm). Lastly, lower real time cell migration speed of 9 ± 1.2 μm / hour also suggests improved attachment of cells on NaOH-etched titanium with values speeds on the polished surface being 65 ± 19 μm / hour.
Surendra Tewari, PhD (Committee Chair)
Joanne Belovich, PhD (Committee Member)
Ronald Midura, PhD (Committee Member)
145 p.
Biomedical Engineering
marrow stromal cells; titanium implant; nano-texture; NaOH-etched surface; cellular attachment; shape factor; live cell imaging; micro-incubator; osteoblast cells; cell migration; cell morphology; aspect ratio; in vitro; green fluorescent protein

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Citations

  • Jensen, R. L. (2013). Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419

    APA Style (7th edition)

  • Jensen, Rebecca. Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model. 2013. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419.

    MLA Style (8th edition)

  • Jensen, Rebecca. "Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model." Master's thesis, Cleveland State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419

    Chicago Manual of Style (17th edition)

csu1391128419
115
© 2013, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.