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Yang_Song_thesis_Final_4_25_2014.pdf (1.64 MB)

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Electrostatically controlled enzymatic reaction, metabolic processes and microbial generation of electric power

Song, Yang
http://rave.ohiolink.edu/etdc/view?acc_num=csu1398685271

Abstract Details

2014, Master of Science in Electrical Engineering, Cleveland State University, Washkewicz College of Engineering.
This thesis shows that electric fields can be used to control electron transport in biological systems and improve the performance of biological devices using an external gating voltage. It has recently been shown that an ultrasensitive detection method based on electric field effect can be used to control the kinetics of enzymatic conversion of glucose. The first part of the thesis is a mechanistic study of the enzyme catalyzed conversion of glucose to gluconolactone using the field effect enzymatic detection (FEED) technique. Use of a voltage controlled enzymatic system decreased electrons tunnel between electrode and active center of glucose oxidase (GOx) to 13mM, as compared with normal status (56mM). The catalytic constant of glucose molecules was also increased to 870S-1. A high concentration of PBS in the system would induce more electrical charges which will induce a stronger electrical field. The results indicate that electrostatic field effect can be used to improve the activity of a redox enzyme immobilized on an electrode by setting up electric field on the interface. Next, the FEED technique is applied to the metabolism of glucose by yeast cells. A transistor-like electrochemical device is constructed to control the glucose metabolism process. Finally, the FEED is used to enhance the performance of yeast-based microbial fuel cell.
SiuTung Yau, PhD (Committee Chair)
Fuqin Xiong, PhD (Committee Member)
Lili Dong, PhD (Committee Member)
Cellular Biology; Electrical Engineering

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Citations

  • Song, Y. (2014). Electrostatically controlled enzymatic reaction, metabolic processes and microbial generation of electric power [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1398685271

    APA Style (7th edition)

  • Song, Yang. Electrostatically controlled enzymatic reaction, metabolic processes and microbial generation of electric power. 2014. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1398685271.

    MLA Style (8th edition)

  • Song, Yang. "Electrostatically controlled enzymatic reaction, metabolic processes and microbial generation of electric power." Master's thesis, Cleveland State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=csu1398685271

    Chicago Manual of Style (17th edition)

csu1398685271
477
© 2014, all rights reserved.
This open access ETD is published by Cleveland State University and OhioLINK.