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Determining the Transport Enhancement of Sodium Fluorescein in Mechanically-Loaded Canine Tibia

Zak, Andrew Joseph
http://rave.ohiolink.edu/etdc/view?acc_num=csu1449314893

Abstract Details

2015, Master of Science in Chemical Engineering, Cleveland State University, Washkewicz College of Engineering.
Quantitative research concerning the impact of mechanical loading on the transport properties of bone has several critical applications. One such application is the effect of a microgravity environment, where the lack of mechanical forces on bone has been shown to negatively impact both growth and repair. A method has been developed in our lab that can potentially allow for the measurement of the effective permeability of large molecules (i.e., 300-15,000 Da in size) in bone tissue under both unloaded and mechanically loaded conditions. In proof-of-concept experiments, previous students have measured the effective diffusivity of the model solute, sodium fluorescein (376 Da) in a sample of unloaded bone tissue. A mechanical loading system has been modified to measure the effective permeability of sodium fluorescein for a bone beam undergoing four point bend testing in a bioreactor system in order to quantify the effect of mechanical loading on solute transport. The first goal of the present work was to validate that deflection of the bone beam was occurring at applied displacements of less than 40 µm. Once the deflection of the bone beam in the mechanical loading system was validated, the primary objective of measuring the transport parameter for sodium fluorescein in canine cortical bone under unloaded and loaded conditions could be achieved. The average value and standard error of this parameter for loaded samples was determined to be 3.70x10-8±1.31x10-8 cm2s-1 (n=5), and 6.59x10-9±2.46x10-9 cm2s-1 (n=4) for unloaded samples. Although a student’s t-test showed that the loaded and unloaded values were not statistically different (p=0.08), this is likely due to the small number of samples. These preliminary results do show that the transport parameter of sodium fluorescein in cortical bone increased by more than factor of 5 with the addition of mechanical loading.
Joanne Belovich, Ph.D. (Committee Chair)
Caroline Androjna, D.Eng (Committee Member)
Surendra Tewari, Ph.D. (Committee Member)
Ronald Midura, Ph.D. (Committee Member)
Biomedical Engineering; Chemical Engineering

Recommended Citations

Citations

  • Zak, A. J. (2015). Determining the Transport Enhancement of Sodium Fluorescein in Mechanically-Loaded Canine Tibia [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1449314893

    APA Style (7th edition)

  • Zak, Andrew. Determining the Transport Enhancement of Sodium Fluorescein in Mechanically-Loaded Canine Tibia. 2015. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1449314893.

    MLA Style (8th edition)

  • Zak, Andrew. "Determining the Transport Enhancement of Sodium Fluorescein in Mechanically-Loaded Canine Tibia." Master's thesis, Cleveland State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=csu1449314893

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Cleveland State University and OhioLINK.