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Investigation into the phase separation behavior of concentrated elastin-like polypeptide solutions

WAN, JIA, HONG
http://rave.ohiolink.edu/etdc/view?acc_num=csu1471923273

Abstract Details

2016, Master of Science in Biomedical Engineering, Cleveland State University, Washkewicz College of Engineering.
Elastin-like polypeptides (ELPs) are environmentally responsive biopolymers that respond to various forms of external stimuli such as temperature, light, and pH. Several factors can influence the transitioning behavior of ELPs. These factors include the amino acid composition of the ELP, the protein concentration in solution, the salt concentration of the solution, and the polymer chain length. Elastin-like polypeptides are soluble in water below a critical solution temperature, however, above this temperature the ELPs become insoluble and phase separate. This point of temperature triggered phase separation is referred to as the transition temperature. This process is completely reversible and ELPs will redissolve once the solution temperature is decreased below the transition temperature. In this study, the phase transitioning behavior for several different concentrations of a three-armed ELP, (GVGVP)40-foldon, was investigated. After expression and purification of this ELP construct, characterization was performed starting at a highly concentrated protein solution then diluting stepwise. Transition temperature were determined using UV-Visible spectrophotometry then several calculation methods were used to analyze the transition temperature data, resulting in multiple potential phase diagrams. Finally, to further investigate the phase transitioning behavior of ELPs microscopy was used to observe the phase transitioning process for several samples of different ELP concentration.
Nolan B Holland, Ph.D. (Advisor)
Moo-Yeal Lee, Ph.D. (Committee Member)
Rolf Lustig, DRE. (Committee Member)
96 p.
Biomedical Engineering; Polymers
Elastin-like polypeptides; transition temperature; phase transition behavior; phase diagram

Recommended Citations

Citations

  • WAN, HONG, J. (2016). Investigation into the phase separation behavior of concentrated elastin-like polypeptide solutions [Master's thesis, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1471923273

    APA Style (7th edition)

  • WAN, HONG, JIA. Investigation into the phase separation behavior of concentrated elastin-like polypeptide solutions. 2016. Cleveland State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1471923273.

    MLA Style (8th edition)

  • WAN, HONG, JIA. "Investigation into the phase separation behavior of concentrated elastin-like polypeptide solutions." Master's thesis, Cleveland State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=csu1471923273

    Chicago Manual of Style (17th edition)

csu1471923273
766
© 2016, some rights reserved.Creative Commons License Investigation into the phase separation behavior of concentrated elastin-like polypeptide solutions by JIA WAN HONG is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Cleveland State University and OhioLINK.