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Application of Advanced Analytical Technologies to Drug Development Studies and Cancer Detection

Voggu, Ramakrishna Reddy
http://rave.ohiolink.edu/etdc/view?acc_num=csu1472677676

Abstract Details

2016, Doctor of Philosophy in Clinical-Bioanalytical Chemistry, Cleveland State University, College of Sciences and Health Professions.
In the past decade, bioanalytical method development has become an integral part of the clinical diagnosis, biomarker discovery, and drug discovery and development. The new and emerged bioanalytical techniques allow the quantitative and qualitative analysis of bio molecules with remarkably high sensitivity and specificity. Specifically, these bioanalytical methods based on LC-MS and methylation-specific PCR are well suited for detecting low-abundance metabolites in various biological fluids and DNA in plasma and tissues for biomarker investigation. They offer great clinical promise for early disease diagnosis and therapeutic intervention. This dissertation broadly organized into two parts, part one talks about the application of LC-MS/MS for drug studies and part two converses about cancer diagnostics using PCR. Part I, The LC-MS/MS quantitative method is essential for the study of pharmacokinetic and toxicological properties in drug screening. Dependent on the type of molecule analyzed, different methods were established to achieve accurate and reliable detection. LC-MS/MS methods were developed and validated for quantitative analysis of an anti-cancer agent CSUOH0901, and an anti-parasitic agent BMCL26. Part II, a Panel of methylated DNA biomarkers for early detection of hepatocellular carcinoma (HCC) was developed by using methylation-specific PCR method. We also developed combination biomarkers for HCC screening. This marker combination enabled sensitive and specific detection of DNA hypermethylation on several tumor-associated genes. The studies with FFPE tissue samples successfully differentiated between HCC and normal tissue samples.
Baochuan Guo, Ph.D. (Advisor)
Bin Su, Ph.D. (Committee Member)
Xiang Zhou, Ph.D. (Committee Member)
Aimin Zhou, Ph.D. (Committee Member)
Chandrasekhar Rama Kothapalli, Ph.D. (Committee Member)
235 p.
Analytical Chemistry

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Citations

  • Voggu, R. R. (2016). Application of Advanced Analytical Technologies to Drug Development Studies and Cancer Detection [Doctoral dissertation, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu1472677676

    APA Style (7th edition)

  • Voggu, Ramakrishna Reddy. Application of Advanced Analytical Technologies to Drug Development Studies and Cancer Detection. 2016. Cleveland State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu1472677676.

    MLA Style (8th edition)

  • Voggu, Ramakrishna Reddy. "Application of Advanced Analytical Technologies to Drug Development Studies and Cancer Detection." Doctoral dissertation, Cleveland State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=csu1472677676

    Chicago Manual of Style (17th edition)

csu1472677676
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© 2016, some rights reserved.Creative Commons License Application of Advanced Analytical Technologies to Drug Development Studies and Cancer Detection by Ramakrishna Reddy Voggu is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Cleveland State University and OhioLINK.