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NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION

Kalil, Haitham Fawzy Mohamed
http://orcid.org/0000-0002-5140-1504
http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904

Abstract Details

2017, Doctor of Philosophy in Clinical-Bioanalytical Chemistry, Cleveland State University, College of Sciences and Health Professions.
Peroxynitrite (ONOO-, PON) plays a crucial role in several cardiovascular dysfunctions and other diseases triggered by oxidative stress. PON is a strong oxidizing agent produced from the diffusion-controlled reaction between nitric oxide radical and superoxide anion-radical. It is also a member of the reactive oxygen-nitrogen species family, which attacks vital components inside the body and initiates deleterious effects via direct and indirect interactions. PON reacts directly with lipids, DNA, and proteins, whereas indirectly, it acts as an initiator of radical-chain reactions. In this work, we have explored various interfaces of manganese-oxide-decorated graphene/hemin and selenium-containing compound for PON detection and quantification. The combination of manganese oxide nanoparticles with the graphene/hemin matrix has allowed for more hemin molecules to be adsorbed on the final composite matrix. As a result, the adsorbed hemin has enhanced the catalytic activity of the final composite and improved the sensitivity towards PON detection. In the same context, a selenium-containing compound (aniline-selenide) has been synthesized and grafted on the electrode surface using the chemistry of diazonium salt. The aniline-selenide-modified electrode showed an increase of approximately 40 times the catalytic current as the aniline-modified electrode. Throughout this project, the preparation methods of the electroactive nanomaterials were described in detail. Moreover, the characterizations of the prepared-materials have been investigated by various physicochemical methods using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), ultraviolet/visible measurements (UV/Vis), and X-ray photoelectron spectroscopy (XPS). This study showed the importance of using selenium and manganese interfaces as sensitive platforms for PON detection. It also provides the initial stage to extend the use of these interfaces to ultramicroelectrodes sensors for use at the level of single cells.
Mekki Bayachou, Ph.D. (Committee Chair)
Aimin Zhou, Ph.D. (Committee Member)
John Turner, Ph.D. (Committee Member)
W. Christopher Boyd, Ph.D. (Committee Member)
Petru S. Fodor, Ph.D. (Committee Member)
111 p.
Chemistry
Peroxynitrite; Cyclic Voltammetry; Manganese functionalized graphene-hemin; Aniline selenide

Recommended Citations

Citations

  • Kalil, H. F. M. (2017). NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION [Doctoral dissertation, Cleveland State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904

    APA Style (7th edition)

  • Kalil, Haitham. NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION. 2017. Cleveland State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904.

    MLA Style (8th edition)

  • Kalil, Haitham. "NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION." Doctoral dissertation, Cleveland State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu151336709631904

    Chicago Manual of Style (17th edition)

csu151336709631904
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© 2017, some rights reserved.Creative Commons License NANOMATERIALS-BASED SENSORS FOR PEROXYNITRITE DETECTION AND QUANTIFICATION by Haitham Fawzy Mohamed Kalil is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Cleveland State University and OhioLINK.