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dayton1229709260.pdf (1.22 MB)
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FUNCTIONAL TESTS OF β TUBULINS IN DROSOPHILA SPERM TAIL MORPHOLOGY
Author Info
Washington, Ashley L.
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1229709260
Abstract Details
Year and Degree
2008, Master of Science (M.S.), University of Dayton, Biology.
Abstract
Drosophilid Beta 2 tubulin has not evolved in 60 million years; variant forms of the protein cannot support spermtail function. Here we test if a unique synergism among amino acids 29, 55, and 57 underlies Beta 2’s function and stasis, by transforming flieswith a chimeric gene consisting of Beta 1 tubulin with Beta 2 codons exchanged at these sites. Sperm of transformed flies undergo tests for fertility, motility and cross-sectional ultrastructure to determine if the chimeric gene supports Beta 2 function. Though transformed flies were not recovered, a second project in related Dipterans suggests synergism may underlie Beta 2’s evolutionary stasis. Mosquito Beta 2, and the major Beta 1 and Alpha 1 tubulins, are evolving rapidly in Anopheles mosquitoes. Anopheles mosquitoes have a 9 + 1, rather than 9 + 2 axoneme, possibly relaxing requirements for tubulin function. Alternatively, absence of the Beta 2 synergism may release tubulin evolution.
Committee
Mark Nielsen, Ph.D. (Advisor)
Albert Burky, Ph.D. (Committee Member)
Sudhindra Gadagkar, Ph.D. (Committee Member)
Pages
77 p.
Subject Headings
Biology
Keywords
Beta tubulin
;
axoneme morphology
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Citations
Washington, A. L. (2008).
FUNCTIONAL TESTS OF β TUBULINS IN DROSOPHILA SPERM TAIL MORPHOLOGY
[Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1229709260
APA Style (7th edition)
Washington, Ashley.
FUNCTIONAL TESTS OF β TUBULINS IN DROSOPHILA SPERM TAIL MORPHOLOGY.
2008. University of Dayton, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1229709260.
MLA Style (8th edition)
Washington, Ashley. "FUNCTIONAL TESTS OF β TUBULINS IN DROSOPHILA SPERM TAIL MORPHOLOGY." Master's thesis, University of Dayton, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1229709260
Chicago Manual of Style (17th edition)
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Document number:
dayton1229709260
Download Count:
593
Copyright Info
© 2008, all rights reserved.
This open access ETD is published by University of Dayton and OhioLINK.