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The antimicrobial effectiveness and cytokine response of Pseudomonas aeruginosa bacteriophages in a human lung tissue culture model

Shiley, Joseph Robert
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1477665544132156

Abstract Details

2016, Master of Science (M.S.), University of Dayton, Biology.
Antibiotic resistance is a major threat to human health. Because of the rise of antibiotic resistant Pseudomonas aeruginosa bacterial infections in cystic fibrosis and other immunocompromised patients, many individuals are left with untreatable and potentially lethal lung infections. Novel methods of treating infections, such as the use of bacteriophages, may provide new means to attenuate these dangerous and oftentimes antibiotic resistant bacteria. The goal of this project was to determine the degree of innate resistance of lung cells to two P. aeruginosa bacteriophages in an in vitro model. The model system used in this project was a human lung tissue co-culture comprised of A549 type II alveolar cells and U937-derived macrophages. In both monoculture and co-culture, it was discovered that after 24 hours, bacteriophage PEV2 induced significant production of IL-8. DMS3 did not yield innate resistance from our panel of four cytokines: IL-6, IL-8, IL-10, and TNF-alpha. Simulated lung infections were carried out with two strains of P. aeruginosa, PAO1 and PAO1-NP, in which the selected bacteriophages demonstrated their ability to protect the A549 monoculture 12 and 24 hours after bacterial exposure.
Jayne Robinson, Ph.D. (Advisor)
Robert Kearns, Ph.D. (Committee Member)
Kristen Comfort, Ph.D. (Committee Member)
Amit Singh, Ph.D. (Committee Member)
84 p.
Biology; Immunology; Microbiology
A549; U937; pseudomonas aeruginosa; tissue culture; cytokine response; IL-6; IL-8; IL-10; TNF-alpha; PAO1; cystic fibrosis; alveolar; co-culture; phage therapy; bacteriophage; innate resistance

Recommended Citations

Citations

  • Shiley, J. R. (2016). The antimicrobial effectiveness and cytokine response of Pseudomonas aeruginosa bacteriophages in a human lung tissue culture model [Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1477665544132156

    APA Style (7th edition)

  • Shiley, Joseph. The antimicrobial effectiveness and cytokine response of Pseudomonas aeruginosa bacteriophages in a human lung tissue culture model. 2016. University of Dayton, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1477665544132156.

    MLA Style (8th edition)

  • Shiley, Joseph. "The antimicrobial effectiveness and cytokine response of Pseudomonas aeruginosa bacteriophages in a human lung tissue culture model." Master's thesis, University of Dayton, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1477665544132156

    Chicago Manual of Style (17th edition)

dayton1477665544132156
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© 2016, all rights reserved.
This open access ETD is published by University of Dayton and OhioLINK.