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Methods of blocking efflux pump activity in Escherichia coli

Auer, Erich Nicholaus
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1524660216368043

Abstract Details

2018, Master of Science (M.S.), University of Dayton, Chemistry.
Overuse of antibiotics throughout the developed world has contributed significantly to the emergence of antibiotic-resistance in bacteria. Although this helps bacteria it is extremely harmful to humans. As bacteria adapt to the antibiotics, current treatments become ineffective and bacterial infections can become life threatening. One of the mechanisms that bacteria, such as E. coli, use are efflux pumps. Efflux pumps are capable of taking potentially harmful substances, like antibiotics, from inside the bacterial cell to outside of the cell. This allows the bacterium to escape from the damaging effects of the antibiotics. My hypothesis was that natural products, small molecule compounds, or DNA aptamers would be able to bind to some part of the tripartite structure of the AcrA-AcrB –TolC efflux pump and block its activity. The use of natural products and small molecule compounds as potential inhibitors allowed me to determine what substances and structural targets are not viable options. The use of DNA aptamers did show significant potential in blocking efflux pump activity.
Matthew Lopper (Advisor)
Justin Biffinger (Committee Member)
Doug Daniels (Committee Member)
45 p.
Biochemistry; Chemistry

Recommended Citations

Citations

  • Auer, E. N. (2018). Methods of blocking efflux pump activity in Escherichia coli [Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1524660216368043

    APA Style (7th edition)

  • Auer, Erich. Methods of blocking efflux pump activity in Escherichia coli. 2018. University of Dayton, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1524660216368043.

    MLA Style (8th edition)

  • Auer, Erich. "Methods of blocking efflux pump activity in Escherichia coli." Master's thesis, University of Dayton, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1524660216368043

    Chicago Manual of Style (17th edition)

dayton1524660216368043
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This open access ETD is published by University of Dayton and OhioLINK.