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Metabolic and Physiological Determinants in Listeria monocytogenes Anaerobic Virulence Regulation

Wallace, Nathan Christopher
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1543424768026244

Abstract Details

2018, Doctor of Philosophy (Ph.D.), University of Dayton, Biology.
In order for enteric pathogens to be successful in causing their infection they must overcome various environmental factors including: low pH, decreased oxygen, high osmolarity, nutrient competition, and host immune system etc. One such pathogen, Listeria monocytogenes (L. monocytogenes), is well known for being extremely adaptable and avoids host immune defenses by causing its infection intracellularly. This unique infection route makes L. monocytogenes an attractive pathogen for gaining further understanding host immune responses to intracellular pathogens. However, the majority of studies involving L. monocytogenes take place in the presence of oxygen, which completely omits the anaerobic phase of gastrointestinal infection. The main goal of this study is to understand the effects of anaerobic growth on L. monocytogenes pathogenesis. Chapter I provides a brief introduction including background information on L. monocytogenes discovery, lifestyle, outbreaks, and pathogenesis. Following the introduction in Chapter II, I give a review on the role of oxygen in the pathogenesis of various other relevant enteric pathogens. Moving into Chapter III, I look at key morphological differences between aerobic and anaerobic growth conditions and explores how exogenous supplementation of key intermediates of the tricarboxylic acid cycle (TCA) affects subsequent pathogenesis. This transitions into Chapter IV, where I explore the role of respiratory activity in priming and sustaining intracellular pathogenesis. And finally, Chapter V evaluates the importance of menaquinone biosynthesis in L. monocytogenes growth and subsequent pathogenesis. Together these data support a regulatory role for metabolic activity in the success of L. monocytogenes infection.
Yvonne Sun, Ph.D. (Advisor)
Amit Singh, Ph.D. (Committee Member)
Jayne Robinson, Ph.D. (Committee Member)
Pothitos Pitychoutis, Ph.D. (Committee Member)
Donald Comfort, Ph.D. (Committee Member)
171 p.
Biology; Microbiology
Listeria; anaerobic; metabolism; respiration; virulence; intracellular growth; bacterial physiology

Recommended Citations

Citations

  • Wallace, N. C. (2018). Metabolic and Physiological Determinants in Listeria monocytogenes Anaerobic Virulence Regulation [Doctoral dissertation, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1543424768026244

    APA Style (7th edition)

  • Wallace, Nathan. Metabolic and Physiological Determinants in Listeria monocytogenes Anaerobic Virulence Regulation. 2018. University of Dayton, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1543424768026244.

    MLA Style (8th edition)

  • Wallace, Nathan. "Metabolic and Physiological Determinants in Listeria monocytogenes Anaerobic Virulence Regulation." Doctoral dissertation, University of Dayton, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1543424768026244

    Chicago Manual of Style (17th edition)

dayton1543424768026244
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© 2018, all rights reserved.
This open access ETD is published by University of Dayton and OhioLINK.