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The Effects of Short Chain Fatty Acids and Oxygen Levels on Listeria Monocytogenes Pathogenesis

Rinehart, Erica Marie
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588239009629426

Abstract Details

2020, Doctor of Philosophy (Ph.D.), University of Dayton, Biology.
Listeria monocytogenes is Gram positive foodborne pathogen that secretes listeriolysin O (LLO), a pore-forming toxin to establish its intracellular life cycle inside host cells. However, how the environment in the intestine that has both an oxygen gradient (low oxygen to no oxygen) and is enriched in short chain fatty acids (SCFAs) affects L. monocytogenes virulence regulation and pathogenesis has not been determined. Thus, the main goal of my thesis research is to understand the effects of anaerobicity and SCFA mixtures on L. monocytogenes pathogenesis. Chapter I compiles and summarizes current findings on the production, mechanisms of action, and potential health benefits of the SCFA propionate. Following this review, Chapter II explores the effect of propionate on growth, membrane composition, and hemolytic activity of L. monocytogenes under aerobic or anaerobic iv conditions. We further tested the effects of SCFA mixtures on the pathogenesis of two different strains of L. monocytogenes (10403s and 07PF0776) in Chapter III to better establish the relevance of our findings. Finally, Chapter IV includes preliminary in vivo results from a young and aged mouse model of infection to determine the effects of SCFAs on dissemination of L. monocytogenes strains 10403s and 07PF0776 after oral inoculation. Together these findings support that L. monocytogenes responds in a straindependent manner to SCFAs and oxygen exposure and alters the outcomes of subsequent in vitro and in vivo infections.
Yvonne Sun (Committee Chair)
Jayne Robinson (Committee Member)
Amit Singh (Committee Member)
Pothitos Pitychoutis (Committee Member)
Kristen Krupa-Comfort (Committee Member)
156 p.
Biology; Microbiology
Listeria monocytogenes; LLO; SCFA; Propionate; Infection

Recommended Citations

Citations

  • Rinehart, E. M. (2020). The Effects of Short Chain Fatty Acids and Oxygen Levels on Listeria Monocytogenes Pathogenesis [Doctoral dissertation, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588239009629426

    APA Style (7th edition)

  • Rinehart, Erica. The Effects of Short Chain Fatty Acids and Oxygen Levels on Listeria Monocytogenes Pathogenesis. 2020. University of Dayton, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588239009629426.

    MLA Style (8th edition)

  • Rinehart, Erica. "The Effects of Short Chain Fatty Acids and Oxygen Levels on Listeria Monocytogenes Pathogenesis." Doctoral dissertation, University of Dayton, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588239009629426

    Chicago Manual of Style (17th edition)

dayton1588239009629426
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This open access ETD is published by University of Dayton and OhioLINK.