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Charles_Brookshire_Thesis_Format_corrected_final format approved LW 4-30-20.pdf (19.02 MB)
ETD Abstract Container
Abstract Header
Illumination Recovery For Optical Microscopy
Author Info
Brookshire, Charles Thomas
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588936914060945
Abstract Details
Year and Degree
2020, Master of Science (M.S.), University of Dayton, Electrical Engineering.
Abstract
We propose a novel solution to the correction of illumination non-uniformity without removing the imaging sample. Calibration of the spatial illumination pattern in microscopy is challenging due to the fact that the illumination source is colocated with the objective lens and therefore cannot be observed directly. Our proposed methodology overcomes this by collecting three spatially translated images in a strategic way. We prove that ``log-illumination pattern'' recovery can be reformulated as a deconvolution of log-ratio of captured images, and develop an efficient, noise-robust implementation. Experiments with simulated and reflectance microscopy data verifies the effectiveness of our proposed approach.
Committee
Keigo Hirakawa, PhD (Advisor)
Bradley Ratliff, PhD (Committee Member)
Michael Uchic, PhD (Committee Member)
Pages
30 p.
Subject Headings
Electrical Engineering
;
Scientific Imaging
Keywords
Non-uniformity correction, Microscopy, Signal Processing, Image Processing, Signal Recovery
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Citations
Brookshire, C. T. (2020).
Illumination Recovery For Optical Microscopy
[Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588936914060945
APA Style (7th edition)
Brookshire, Charles.
Illumination Recovery For Optical Microscopy .
2020. University of Dayton, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588936914060945.
MLA Style (8th edition)
Brookshire, Charles. "Illumination Recovery For Optical Microscopy ." Master's thesis, University of Dayton, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1588936914060945
Chicago Manual of Style (17th edition)
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Document number:
dayton1588936914060945
Download Count:
117
Copyright Info
© 2020, all rights reserved.
This open access ETD is published by University of Dayton and OhioLINK.