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Full text release has been delayed at the author's request until May 07, 2025

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Revealing the Dynamics of the Limb-Brain Axis During Axolotl Limb Regeneration

Tornes, Jason Andrew
http://rave.ohiolink.edu/etdc/view?acc_num=dayton1683214817077628

Abstract Details

2023, Master of Science (M.S.), University of Dayton, Biology.
The critical role that the nervous system plays in driving amphibian limb regeneration underscores the impact that the brain may also have in this fascinating process. Earlier studies have suggested that unique protein synthesis occurs in the brain of regenerating newts upon limb amputation and have also exposed the prominent regenerative potential of brain-derived neural extracts from adult newts and chicken embryos. In the context of the current thesis, we employed a combination of in vivo axolotl limb amputation models, neurochemical and high-throughput proteomics approaches, as well as fluorescent immunohistochemistry to capture the temporal neuromolecular alterations that occur in the brain of regenerating axolotls. In our experimental setup, amputation and subsequent regeneration of the forelimbs affected the central proteomic signatures and neurochemistry in a time-dependent fashion into the regeneration program. To our knowledge this is the first study to systematically investigate the molecular and cellular dynamics underlying the activation of the limb-brain axis during amphibian limb regeneration, laying the groundwork for considering the role of the brain in the regulation of limb regeneration activity.
Pothitos Pitychoutis (Advisor)
Mrigendra Rajput (Committee Member)
Jennifer Hellmann (Committee Member)
Katia Del Rio-Tsonis (Committee Member)
60 p.
Biology; Neurosciences
axolotl; limb regeneration; central nervous system; proteomics; neurotransmitter

Recommended Citations

Citations

  • Tornes, J. A. (2023). Revealing the Dynamics of the Limb-Brain Axis During Axolotl Limb Regeneration [Master's thesis, University of Dayton]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1683214817077628

    APA Style (7th edition)

  • Tornes, Jason. Revealing the Dynamics of the Limb-Brain Axis During Axolotl Limb Regeneration. 2023. University of Dayton, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=dayton1683214817077628.

    MLA Style (8th edition)

  • Tornes, Jason. "Revealing the Dynamics of the Limb-Brain Axis During Axolotl Limb Regeneration." Master's thesis, University of Dayton, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1683214817077628

    Chicago Manual of Style (17th edition)

dayton1683214817077628
© 2023, all rights reserved.
This open access ETD is published by University of Dayton and OhioLINK.