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INNATE AND ADAPTIVE HOST RESPONSE DURING THE INITIAL PHASE OF HERPES SIMPLEX VIRUS ENCEPHALITIS IN THE NEONATAL MOUSE

Kumaraswamy, Guttalu K
http://rave.ohiolink.edu/etdc/view?acc_num=kent1168650862

Abstract Details

2007, PHD, Kent State University, College of Biomedical Sciences.
To study early events of neonatal Herpes Simplex Virus (HSV) encephalitis and its sequelae we induced a controlled infection in the brains of mice using HSVgH-, a genetically modified Disabled Infective Single Cycle virus. Neonatal Balb/C mice were infected with various amounts of HSVgH- virus by intracerebral injection. Results showed that the survival of infected mice was dependent on the amount of virus injected. Infection with 200,000 plaque forming units (pfu) of HSVgH- virus resulted in 0% survival, whereas 25,000 pfu resulted in 75% survival. If the mice died, 98% of the deaths occurred between 3 and 7 days after infection. Replication competent virus was recovered from 20% of mice brains infected with 25,000 pfu of HSVgH-. Neutralizing antibodies were not detected 6 weeks post infection in sera of mice, which survived infection with 25,000 pfu of HSVgH-. LacZ histochemistry and immunoperoxidase staining using anti-HSV and anti-beta-galactosidase antibody revealed that the infection was limited to the site of injection. Tissue destruction was observed at the site of inoculation 3 days post infection using cresyl violet staining. At 3 days post infection adjacent sections showed positive cells for viral antigens and apoptotic cells in the infected area. Immunoperoxidase staining using antibodies to surface markers showed microglial activation beginning on day 1 and astrocyte proliferation beginning on day 3-post infection. B and T lymphocytes were not detected on day 1 through 7-post infection. This controlled experimental HSV infection suggests a limited non-specific early host response of innate immunity and absence of adaptive immunity in the neonate to HSV encephalitis.
John Docherty (Advisor)
infection; NEONATAL; VIRUS; infected; HSV; post infection; HSVgH

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Citations

  • Kumaraswamy, G. K. (2007). INNATE AND ADAPTIVE HOST RESPONSE DURING THE INITIAL PHASE OF HERPES SIMPLEX VIRUS ENCEPHALITIS IN THE NEONATAL MOUSE [Doctoral dissertation, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1168650862

    APA Style (7th edition)

  • Kumaraswamy, Guttalu. INNATE AND ADAPTIVE HOST RESPONSE DURING THE INITIAL PHASE OF HERPES SIMPLEX VIRUS ENCEPHALITIS IN THE NEONATAL MOUSE. 2007. Kent State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1168650862.

    MLA Style (8th edition)

  • Kumaraswamy, Guttalu. "INNATE AND ADAPTIVE HOST RESPONSE DURING THE INITIAL PHASE OF HERPES SIMPLEX VIRUS ENCEPHALITIS IN THE NEONATAL MOUSE." Doctoral dissertation, Kent State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=kent1168650862

    Chicago Manual of Style (17th edition)

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© 2007, all rights reserved.
This open access ETD is published by Kent State University and OhioLINK.