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S-phase Synchronization Promotes Chemoradiotherapy-induced Apoptosis in Prostate Cancer Cell Lines

Shyam, Sunitha
http://rave.ohiolink.edu/etdc/view?acc_num=kent1185835523

Abstract Details

2007, MS, Kent State University, College of Biomedical Sciences.
Prostate cancer is the second leading cause of death in men in the USA for the year 2007 and cells are generally resistant to treatments. Hence it is vital to sensitize them prior to treatment. Synchronizing cells in a particular phase of the cell cycle is a simple and efficient method of sensitization. We used aphidicolin to synchronize cells in S-phase of the cell cycle, thus sensitizing them to therapeutic agents. About ninety percent of prostate cancer cells could be synchronized using this DNA polymerase inhibitor. Synchronized cells, when treated with agents such as Apo2L/TRAIL, CPT-11 and Bortezomib, showed increased levels of apoptosis. Combination treatments enabled greatly to reduce doses of the agents used, with prior synchronization in S-phase. Thus, S-phase synchronization might represent a promising avenue of treatment of tumor cells, which are highly resistant to various chemo-irradiation therapies.
Gail Fraizer (Advisor)
Biology, Molecular
Apo2L/TRAIL; Bortezomib; C4-2; PARP-1; aphidicolin; Cell; C4-2 cells

Recommended Citations

Citations

  • Shyam, S. (2007). S-phase Synchronization Promotes Chemoradiotherapy-induced Apoptosis in Prostate Cancer Cell Lines [Master's thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1185835523

    APA Style (7th edition)

  • Shyam, Sunitha. S-phase Synchronization Promotes Chemoradiotherapy-induced Apoptosis in Prostate Cancer Cell Lines. 2007. Kent State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1185835523.

    MLA Style (8th edition)

  • Shyam, Sunitha. "S-phase Synchronization Promotes Chemoradiotherapy-induced Apoptosis in Prostate Cancer Cell Lines." Master's thesis, Kent State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=kent1185835523

    Chicago Manual of Style (17th edition)

kent1185835523
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© 2007, all rights reserved.
This open access ETD is published by Kent State University and OhioLINK.