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Role of Glycolysis and Respiration in Sperm Metabolism and Motility

Pasupuleti, Vinay
http://rave.ohiolink.edu/etdc/view?acc_num=kent1195536178

Abstract Details

2007, MS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
Mammalian spermatozoa are designed to traverse outside the body to fertilize the egg in the female reproductive tract. There is a need to maintain motility for extended periods of time under varying conditions. In cells, glycolysis and mitochondrial respiration are the two major pathways which provide ATP. The relative contributions of glycolysis and mitochondrial respiration in maintaining mouse sperm motility has been a subject of controversy. Recent research suggests that glycolysis is essential for mouse sperm motility and that mitochondrial respiration may not be necessary. In this study we re-examined the relative roles of glycolysis and mitochondrial respiration in ATP production and sperm motility. This was done using two inhibitors of glycolysis, 2-deoxy-D-glucose (DOG) and iodoacetamide. DOG decreased ATP levels and motility in presence of pyruvate and lactate. However, iodoacetamide, an inhibitor of glyceraldehyde-3-phosphate dehydrogenase, did not reduce motility and ATP levels in presence of pyruvate and lactate. We attribute the inhibitory effect of DOG to utilization of ATP produced by mitochondrial respiration for its own phosphorylation. Thus, DOG drains ATP irrespective of where it comes from. However, iodoacetamide inhibited ATP produced from glycolysis with no effect on ATP produced by mitochondrial respiration. DOG is a weak competitive inhibitor of glucose. Thus, DOG in presence of glucose did not significantly affect ATP levels. DOG caused significant decrease in ATP when fructose was used as a glycolytic substrate. On the other hand, iodoacetamide in presence of glucose significantly reduced ATP levels. Taken together, our data shows that mouse sperm can remain motile with ATP generated from either glycolysis or mitochondrial respiration.
Srinivasan Vijayaraghavan (Advisor)
55 p.
Biology, Molecular
spermatozoa; metabolism; glycolysis; respiration

Recommended Citations

Citations

  • Pasupuleti, V. (2007). Role of Glycolysis and Respiration in Sperm Metabolism and Motility [Master's thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1195536178

    APA Style (7th edition)

  • Pasupuleti, Vinay. Role of Glycolysis and Respiration in Sperm Metabolism and Motility. 2007. Kent State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1195536178.

    MLA Style (8th edition)

  • Pasupuleti, Vinay. "Role of Glycolysis and Respiration in Sperm Metabolism and Motility." Master's thesis, Kent State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=kent1195536178

    Chicago Manual of Style (17th edition)

kent1195536178
10,656
© 2007, all rights reserved.
This open access ETD is published by Kent State University and OhioLINK.