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Phosphorylation-dependent interaction of tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein (14 3-3) with PADI6 following oocyte maturation in mice

Snow, Alan J.
http://rave.ohiolink.edu/etdc/view?acc_num=kent1208796428

Abstract Details

2008, PHD, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
Proteins in the tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (14-3-3) family are involved in the regulation of many intracellular processes. We have examined the interaction of 14-3-3 with peptidylarginine deiminase, type VI (PADI6), an abundant protein found in mammalian oocytes, eggs and early embryos. Peptidylarginine deiminases catalyze the posttranslational modification of peptidylarginine to citrulline. PADI6 is associated with oocyte cytoplasmic sheets containing intermediate filaments and it has been shown that PADI6-deficient mice are infertile due to disruption of development beyond the two-cell stage. We found that PADI6 undergoes a dramatic developmental change in phosphorylation during oocyte maturation. This change in phosphorylation is linked to an interaction of PADI6 with 14-3-3 in the mature egg. Recombinant glutathione-s-transferase (GST) 14-3-3 pull-down experiments and transgenic tandem affinity purification with liquid chromatography/mass spectrometry demonstrates a binding interaction between 14-3-3 and PADI6 in mature eggs, but not in the immature oocytes. 14-3-3 proteins modulate or complement intracellular events involving phosphorylation dependent switching or protein modification. These results indicate that phosphorylation and/or 14-3-3 binding may serve as a means of intracellular PADI6 regulation.
Douglas Kline, PhD (Committee Chair)
108 p.
Biology
oocyte; egg; ovum; PADI6; PAD6; 14-3-3; peptidylarginine deiminase; phosphorylation; mouse; oocyte maturation; gamete biology

Recommended Citations

Citations

  • Snow, A. J. (2008). Phosphorylation-dependent interaction of tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein (14 3-3) with PADI6 following oocyte maturation in mice [Doctoral dissertation, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1208796428

    APA Style (7th edition)

  • Snow, Alan. Phosphorylation-dependent interaction of tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein (14 3-3) with PADI6 following oocyte maturation in mice. 2008. Kent State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1208796428.

    MLA Style (8th edition)

  • Snow, Alan. "Phosphorylation-dependent interaction of tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein (14 3-3) with PADI6 following oocyte maturation in mice." Doctoral dissertation, Kent State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=kent1208796428

    Chicago Manual of Style (17th edition)

kent1208796428
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© 2008, all rights reserved.
This open access ETD is published by Kent State University and OhioLINK.