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Relative Resistin Expression in Normally Cycling and Cystic Rat Ovaries

Grampa, Allison Mary
http://rave.ohiolink.edu/etdc/view?acc_num=kent1277429991

Abstract Details

2010, MS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
Resistin is a recently identified hormone which may play a role in insulin resistance, inflammation, human polycystic ovary syndrome, or other physiological states. Resistin protein has been identified and localized in rat testes and the gene for resistin has been identified in mouse ovaries. This led to the hypotheses that resistin mRNA and protein may be present in normally cycling rat ovaries and may differ in ovaries of an animal model of human polycystic ovary syndrome. Rats in the animal model of polycystic ovary syndrome were hypothyroid and treated with hCG to induce ovarian cysts. Resistin mRNA expression was quantified using real-time PCR. Resistin mRNA was present in the ovaries of normally cycling rats in all stages of the estrous cycle, as well as in those of an animal model of human polycystic ovary syndrome. Resistin mRNA did not significantly differ between stages of the cycle. Resistin mRNA was also not different when comparing ovaries of normally cycling rats and cystic ovaries. Resistin protein expression and localization were attempted using Western blot and immunohistochemistry. Resistin protein was not detected with confidence using either of these methods. Until the receptor and signaling pathway for resistin have been elucidated, it will remain difficult to study this molecule.
Jennifer Marcinkiewicz, PhD (Advisor)
Brent Bruot, PhD (Committee Member)
Gail Fraizer, PhD (Committee Member)
94 p.
Biology
resistin ovary

Recommended Citations

Citations

  • Grampa, A. M. (2010). Relative Resistin Expression in Normally Cycling and Cystic Rat Ovaries [Master's thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1277429991

    APA Style (7th edition)

  • Grampa, Allison. Relative Resistin Expression in Normally Cycling and Cystic Rat Ovaries. 2010. Kent State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1277429991.

    MLA Style (8th edition)

  • Grampa, Allison. "Relative Resistin Expression in Normally Cycling and Cystic Rat Ovaries." Master's thesis, Kent State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=kent1277429991

    Chicago Manual of Style (17th edition)

kent1277429991
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© 2010, all rights reserved.
This open access ETD is published by Kent State University and OhioLINK.