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kent1322501214.pdf (10.5 MB)
ETD Abstract Container
Abstract Header
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory
Author Info
Bhatta, Fanindra P.
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214
Abstract Details
Year and Degree
2011, PHD, Kent State University, College of Arts and Sciences / Department of Physics.
Abstract
This dissertation looks at the phase-separation of two binary lipid systems: (a) an experimental study of binary lipid Langmuir monolayers and (b) the theoretical modeling of a binary system of lipids in a symmetric bilayer. Experimentally, monolayers of dihydrocholesterol (Dchol) and dimyristoylphosphatidylcholine (DMPC) at the air/water interface were studied with Brewster angle microscopy (BAM) and fluorescence microscopy (FM) in order to test the effect of fluorescent dyes, which may be strongly line active, on the phase diagram. The theoretical model of phase separation in mixed lipid bilayers emphasizes the asymmetry of the two layers of a bilayer. A Landau free energy in terms of order parameters the cholesterol mole fraction, and the hydrocarbon chain length is extended to look at both cubic and quartic terms. We found four states: (1) a single homogeneous phase, (2) two-phase coexistence, (3) three-phase coexistence, and (4) four-phase coexistence, depending on temperature and the composition of the layers. Three phase and four phase coexistence are of special interest because they have been little studied either theoretically or experimentally.
Committee
David Allender, Dr. (Committee Chair)
Elizabeth Mann, Dr. (Committee Co-Chair)
John Portman, Dr. (Committee Member)
Edgar Kooijman, Dr. (Committee Member)
Anatoly Khitrin, Dr. (Committee Member)
Pages
263 p.
Subject Headings
Biophysics
;
Physics
;
Solid State Physics
Keywords
Phase separation
;
mixed lipid monolayers and bilayers
;
Brewster angle microscopy
;
fluorescence microscopy
;
Landau free energy
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Citations
Bhatta, F. P. (2011).
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory
[Doctoral dissertation, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214
APA Style (7th edition)
Bhatta, Fanindra.
Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory.
2011. Kent State University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214.
MLA Style (8th edition)
Bhatta, Fanindra. "Phase Separation in Binary Lipid Monolayers Bilayers: Experiment and Theory." Doctoral dissertation, Kent State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=kent1322501214
Chicago Manual of Style (17th edition)
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Document number:
kent1322501214
Download Count:
1,111
Copyright Info
© 2011, all rights reserved.
This open access ETD is published by Kent State University and OhioLINK.