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Evidence for the Role of YWHA in Mouse Oocyte Maturation

Detwiler, Ariana Claire

Abstract Details

2015, MS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
Processes essential to the growth and differentiation of oocytes, such as meiosis and spindle formation, are dependent upon intricate interactions between key proteins. The Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation (YWHA) family of proteins have been found to be essential to vertebrate cell cycle regulation and development. There are seven isoforms, each encoded by a different gene. Previous studies have shown that these proteins are involved in maintenance of prophase I arrest in the GV-intact oocyte, as well as proper spindle formation. Real-time and quantitative PCR methods were employed to study the relative levels of expression of these proteins’ transcripts in the mouse oocyte and egg. Protein pull-down experiments were also used to investigate the interaction of the key phosphatase in meiosis resumption, CDC25B, and the YWHAH protein in oocytes and eggs. PCR results confirm the presence of at least six of the seven isoforms in both oocytes and eggs of two strains of mice, and suggest a decrease in relative expression of the transcripts through oocyte maturation. The pull-down results confirm the binding and interaction of YWHAH with CDC25B. This study increases the knowledge available on the YWHA proteins, and provides a strong basis for further studies.
Douglas Kline (Advisor)
Srinivasan Vijayaraghavan (Committee Member)
Marcinkiewicz Jennifer (Committee Member)

Recommended Citations

Citations

  • Detwiler, A. C. (2015). Evidence for the Role of YWHA in Mouse Oocyte Maturation [Master's thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1438019442

    APA Style (7th edition)

  • Detwiler, Ariana. Evidence for the Role of YWHA in Mouse Oocyte Maturation. 2015. Kent State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1438019442.

    MLA Style (8th edition)

  • Detwiler, Ariana. "Evidence for the Role of YWHA in Mouse Oocyte Maturation." Master's thesis, Kent State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=kent1438019442

    Chicago Manual of Style (17th edition)