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Ionization Properties of Diacylglycerol-pyrophosphate and Phosphatidylinositol 3,4,5-trisphosphate in Model Membranes

Thomas, Joseph
http://rave.ohiolink.edu/etdc/view?acc_num=kent1472936455

Abstract Details

2016, MS, Kent State University, College of Arts and Sciences / School of Biomedical Sciences.
Diacylglycerol-pyrophosphate (DGPP) is a component of plant cell membranes that is involved in stress signaling. The enzyme that produces DGPP (PA-kinase) is unknown and the exact mechanism by which DGPP acts during stress signaling needs to be resolved. This work aims to shed some light on the ionization behavior of DGPP, as it negative charge affects how it will interact with proteins. The ionization behavior of DGPP was probed using nuclear magnetic resonance (NMR) spectroscopy using model membranes containing DGPP, phosphatidic acid (PA), phosphatidylcholine (PC), and phosphatidylethanolamine (PE). Ionization data was collected over a range of pH values and used to create a titration curve. These curves can then be used to predict the ionization of DGPP at a given pH value. Our NMR data suggests that specific ionization states of DGPP are capable of forming local interactions with PA. Interactions of DGPP with PA in the cell membrane have been explored via the use of fluorescence microscopy. These interactions may be observed as changes in local membrane curvature, or as phase separations that will selectively exclude the fluorescent phospholipid in use. Phosphatidylinositol 3,4,5-trisphosphate (PIP3) is another important membrane phospholipid found in animal cells. PIP3 has been shown to be involved in the regulation of cell growth, and dysregulation of its signaling pathways has been implicated in some forms of cancer. Previous work has shown that the lipid phosphatidylinositol 4,5-bisphosphaste (PIP2), which is structurally similar to PIP3, is capable of interacting with phosphatidylinositol (PI) in model membranes. These interactions alter the charge of PIP2, and similar behavior is observed in model membranes containing PI and PIP3. The ionization of this lipid in model membranes was also investigated via NMR and analyzed in a similar manner as the DGPP data.
Edgar Kooijman, Dr. (Advisor)
Ernest Freeman, Dr. (Committee Member)
Jennifer McDonough, Dr. (Committee Member)
106 p.
Biomedical Research

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Citations

  • Thomas, J. (2016). Ionization Properties of Diacylglycerol-pyrophosphate and Phosphatidylinositol 3,4,5-trisphosphate in Model Membranes [Master's thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1472936455

    APA Style (7th edition)

  • Thomas, Joseph. Ionization Properties of Diacylglycerol-pyrophosphate and Phosphatidylinositol 3,4,5-trisphosphate in Model Membranes. 2016. Kent State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1472936455.

    MLA Style (8th edition)

  • Thomas, Joseph. "Ionization Properties of Diacylglycerol-pyrophosphate and Phosphatidylinositol 3,4,5-trisphosphate in Model Membranes." Master's thesis, Kent State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=kent1472936455

    Chicago Manual of Style (17th edition)

kent1472936455
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This open access ETD is published by Kent State University and OhioLINK.