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INTERACTIONS AND LOCALIZATION OF PROTEIN PHOSPHATASES, YWHA PROTEINS AND CELL CYCLE CONTROL PROTEINS IN MEIOSIS

Gilker, Eva Adeline, Gilker
http://rave.ohiolink.edu/etdc/view?acc_num=kent1532699317257539

Abstract Details

2018, MS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
Oocytes, the female gametes, are produced during oogenesis and held at meiotic prophase I arrest in the ovary until reproductive maturity. In mammals, the oocyte must undergo maturation to differentiate into the fertilizable egg. It is known that a key protein involved in resumption of meiosis is Cell Division Cycle 25B (CDC25B), which dephosphorylates and activates Cyclin-Dependent Kinase I (CDKI). Active CDKI initiates germinal vesicle breakdown (GVBD), the first signal of resumption of meiosis. The tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation proteins (YWHA) are known to be responsible for maintaining arrest through binding CDC25B, rendering it inactive. Here, co-localization analysis and FRET were used to show that YWHAH and YWHAE are able to form heterodimers in the mammalian oocyte. Members of the phosphoprotein phosphatase family of serine/threonine phosphatases (PPP) are also implicated to be involved in regulation of CDC25B. Through various pull down assays, it is observed that PP1¿1 binds CDC25B in the ovary. When PP1¿ is absent, PP1a binds CDC25B, implicating these isoforms may be functionally interchangeable. This works lays important foundations for the future study of the intricate pathway involved in mammalian female meiosis.
Douglas Kline, Dr. (Advisor)
Srinivasan Vijayaraghavan , Dr. (Committee Member)
Kristy Welshhans, Dr. (Committee Member)
73 p.
Biology; Cellular Biology
Oocyte maturation; Meiosis; YWHA; PP1; CDC25B; Oocyte

Recommended Citations

Citations

  • Gilker, Gilker, E. A. (2018). INTERACTIONS AND LOCALIZATION OF PROTEIN PHOSPHATASES, YWHA PROTEINS AND CELL CYCLE CONTROL PROTEINS IN MEIOSIS [Master's thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=kent1532699317257539

    APA Style (7th edition)

  • Gilker, Gilker, Eva. INTERACTIONS AND LOCALIZATION OF PROTEIN PHOSPHATASES, YWHA PROTEINS AND CELL CYCLE CONTROL PROTEINS IN MEIOSIS. 2018. Kent State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=kent1532699317257539.

    MLA Style (8th edition)

  • Gilker, Gilker, Eva. "INTERACTIONS AND LOCALIZATION OF PROTEIN PHOSPHATASES, YWHA PROTEINS AND CELL CYCLE CONTROL PROTEINS IN MEIOSIS." Master's thesis, Kent State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=kent1532699317257539

    Chicago Manual of Style (17th edition)

kent1532699317257539
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This open access ETD is published by Kent State University and OhioLINK.