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Identification of a Culture Cell Model to Study Hemoglobin Expression in Neurons

Bhatta, Sabina
http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1304621619

Abstract Details

2011, BS, Kent State University, College of Arts and Sciences / Department of Chemistry and Biochemistry.
An inflammatory neurodegenerative disease, Multiple Sclerosis (MS), affects the central nervous system and results in a progressive physical and cognitive disability in the patients. Hemoglobin expression in neurons has been found to be increased in neurons in postmortem MS brain tissue compared to controls. The role of hemoglobin in neurons is unknown but evidence suggests it may be involved in neuronal respiration and may provide protection from the damaging effects of free radicals and reactive oxygen species. The ability to modulate hemoglobin expression in neurons may be beneficial in diseases such as MS where mitochondrial dysfunction and oxidative damage are involved in disease pathology. Both transformed neural cell lines and primary cultured neurons were tested to see if they expressed hemoglobin. Neuroblastoma cells (SHSY-5Y) and neura-2a cells were treated with hydrogen peroxide (H2O2). Then primary neurons were treated with L-methionine (L-met), trichostatin A (TSA), hydrogen peroxide (H2O2), and erythropoietin (EPO). We confirmed that hemoglobin is expressed in primary cultured neurons and the expression of hemoglobin could be modulated in this culture system.
Ernest Freeman, Ph.D (Advisor)
Jennifer McDonough, Ph.D (Advisor)
Gail Fraizer, Ph.D (Committee Member)
Soumitra Basu, Ph.D (Committee Member)
40 p.
Biomedical Research; Neurosciences
Hemoglobin; neurons; MS

Recommended Citations

Citations

  • Bhatta, S. (2011). Identification of a Culture Cell Model to Study Hemoglobin Expression in Neurons [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1304621619

    APA Style (7th edition)

  • Bhatta, Sabina. Identification of a Culture Cell Model to Study Hemoglobin Expression in Neurons. 2011. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1304621619.

    MLA Style (8th edition)

  • Bhatta, Sabina. "Identification of a Culture Cell Model to Study Hemoglobin Expression in Neurons." Undergraduate thesis, Kent State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1304621619

    Chicago Manual of Style (17th edition)

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© 2011, all rights reserved.
This open access ETD is published by Kent State University Honors College and OhioLINK.