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Inhibition of Ovarian Cancer Cell Proliferation by Oleoylethanolamide and its Metabolically Stable Analog AM3102

Kisgeropoulos, Effie Christine
http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1386429690

Abstract Details

2013, BS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
While the acylethanolamide oleoylethanolamide (OEA) has been found in the human ovary, its effect on ovarian cells in particular has not been studied. This study investigated the effects of OEA and its metabolically stable analog AM3102 on the human ovarian cancer cell line OV2008. Cell culture experiments, western blotting, and fluorescence microscopy were performed, using OEA and various other reagents. Possible targets investigated include the phospholipase A2 signaling pathway, OEA cell receptors GPR119 and PPAR-alpha, and reactive oxygen species (ROS) levels. Results indicated that both OEA and AM3102 inhibit cell proliferation and induce apoptosis in OV2008 cells in serum-free medium. Novel evidence was also provided concerning OEA and its targets in the cell, including the possibility that PTK might have cellular targets, besides iPLA2 and cPLA2, which are unknown. Finally, results indicated OEA's involvement in the increase of membrane ROS could be a key factor in cell death, as toxicity was reversible through a-tocopherol (a lipid-soluble antioxidant) but not Trolox (its water-soluble analog). Continued investigation into the details of these signaling cascades is recommended to help to better our understanding of OEA as a possible inhibitor of proliferation in cancerous cells.
P Bagavandoss, Dr. (Advisor)
Robert Hamilton, Dr. (Committee Member)
Leslie Heaphy, Dr. (Committee Member)
Julie Cremeans-Smith, Dr. (Committee Member)
46 p.
Biology; Biomedical Research; Cellular Biology
ovarian cancer; oleoylethanolamide; phospholipase A2; reactive oxygen species; endocannabinoid

Recommended Citations

Citations

  • Kisgeropoulos, E. C. (2013). Inhibition of Ovarian Cancer Cell Proliferation by Oleoylethanolamide and its Metabolically Stable Analog AM3102 [Undergraduate thesis, Kent State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1386429690

    APA Style (7th edition)

  • Kisgeropoulos, Effie. Inhibition of Ovarian Cancer Cell Proliferation by Oleoylethanolamide and its Metabolically Stable Analog AM3102. 2013. Kent State University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1386429690.

    MLA Style (8th edition)

  • Kisgeropoulos, Effie. "Inhibition of Ovarian Cancer Cell Proliferation by Oleoylethanolamide and its Metabolically Stable Analog AM3102." Undergraduate thesis, Kent State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1386429690

    Chicago Manual of Style (17th edition)

ksuhonors1386429690
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© 2013, all rights reserved.
This open access ETD is published by Kent State University Honors College and OhioLINK.